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余飞,吕明丽,蔡海东,等.miR-506在乳腺肿瘤细胞增殖及转移中的作用[J].同济大学学报（医学版）,2013,34(1):25-29. [点击复制]
YU Fei,LUMing-li,CAI Hai-dong,et al.Effect of miR-506 on proliferation and migration of breast cancer cells[J].同济大学学报（医学版）,2013,34(1):25-29. [点击复制]

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miR-506在乳腺肿瘤细胞增殖及转移中的作用
余飞,吕明丽,蔡海东,李丹,袁雪宇,吕中伟
0 字体:加大+\|默认\|缩小-
(同济大学附属第十人民医院核医学科，上海 200072)

摘要:

目的分析miR．506与乳腺癌增殖转移的关系。方法miR-506minics/inhibitor/NC瞬转入MDA—MB一231乳腺癌细胞后进行CCK-8细胞增殖，细胞计数，集落形成，Transwell小室实验及Real—TimePCR。结果miR-506相关核苷酸（mimics、inhibitor、NC）瞬转入细胞后第1天miR-506inhibitor即显现出促进细胞增殖的效应，第3天后miR-506mimics开始出现明显的细胞增殖抑制效应；集落形成实验中miR-506mimics组细胞所形成的集落数量明显少于miR-506 inhibitor组和NC组；Transwell小室实验显示三种miR．506mimics所转染细胞的穿过膜的数量少于miR-506inhibitor组和NC组。Real-TimePCR显示，与miR-506 inhibitor组相比，miR-506mimics组酌5、MMP2基因表达增高，而RASSFl基因降低。结论miR-506过表达可以明显的抑制细胞的增殖能力，单克隆集落形成能力和侵袭转移能力，即miR-506高表达可以抑制乳腺癌细胞的恶性表型。这种作用可能与p65、MMP2及RASSFl表达有关。

关键词: miR-506 乳腺肿瘤细胞增殖

DOI：10.3969/j.issn1008-0392.2013.01.006

基金项目:

Effect of miR-506 on proliferation and migration of breast cancer cells

YU Fei,LUMing-li,CAI Hai-dong,LI Dan,YUAN Xue-yu,LU Zhong-wei

(Dept.of Nuclear Medicine,Tenth People’s Hospital,Tongji University,Shanghai 200072,China)

Abstract:

Objective To invesstigate the effect of miR-506 on proliferation and migration of breast cancer cells. Methods miR-506 related nucleotides （mimics, inhibitor, NC） were transfected to breast cancer MDA-MB-231 cells. The cell proliferation, clone formation test, Transwell test were performed. Results The miR-506 inhibitor promoted cell proliferation on the first day of transfection, while miR- 506 mimics significantly inhibited cell proliferation 3 days after transfection. Clone formation tests demonstrated that the clone number in miR-506 mimics group was less than that of miR-506 inhibitor and NC groups. Transwell test showed that the number of migrated cells in 3 miR-506 mimics groups was less than that of miR-506 inhibitor group and NC group. Real-Time PCR results showed that the levels of p65, MMP2 increased and RASSF1 decreased in miR-506 mimics groups. Conclusion High expression of miR-506 can inhibit cell proliferation, clone formation and cell migration of breast cancer MDA-MB- 231 cells, and the inhibition may related to MMP2, p65 and RASSF1.

Key words: miR-506 breast tumor cell proliferation