| 摘要: |
| 目的探讨罗格列酮（Rosiglitazone，ROZ）体外抑制结肠癌生长的作用。方法体外培养人结肠癌细胞HT-29，不同浓度ROZ（10、20、40、80μmol/L）干预。MTT法测定细胞增殖，透射电镜、TUNEL法及流式细胞术观察细胞凋亡情况，免疫组织化学法检测各组细胞中Bax及Bcl-2的表达。结果MTT法显示ROZ抑制HT-29细胞生长，且具有时间和浓度依赖性，透射电镜、TUNEL法及流式细胞术可观察到ROZ具有诱导结肠癌细胞凋亡的作用，免疫组化法显示经ROZ处理后的结肠癌细胞Bax蛋白表达增强，Bcl-2蛋白表达减弱。结论ROZ体外具有抑制结肠癌细胞生长的作用，与其诱导细胞凋亡有关，可能通过增强Bax的表达及减弱Bcl-2的表达实现。 |
| 关键词: 罗格列酮 结肠肿瘤 增殖 凋亡 |
| DOI：10.3969/j.issn1008-0392.2013.01.005 |
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| 基金项目: |
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| Inhibitory effect of rosiglitazone on colon cancer cells in vitro |
| ZHANG Li1,WANG Zhi-rong2 |
| (1.Dept.of Internal Medicine,Branch of Tongji Hospital,Tongji University,Shanghai 200092,China;;2.Dept.of Gastroenterology,Tongji Hospital,Tongji University,Shanghai 200065,China) |
| Abstract: |
| Objective To investigate the inhibitory effect of Rosiglitazone （ROZ） on colon cancer cells in vitro. Methods Cultured colon cancer HT-29 cells were treated by different concentrations of ROZ （ 10, 20, 40 and 80 μmol/L） in vitro. The inhibitory effect of ROZ on the growth of cells was evaluated by MTT; cell apoptosis was detected with TEM, TUNEL and flow cytometry; the protein expression of Bax and Bcl-2 was detected with immunocytochemistry technique. Results The growth and proliferation of HT-29 cells were inhibited significantly by ROZ in a concentration and time- dependent manner. TEM, TUNEL and flow cytometry revealed that cell apoptosis was induced by ROZ treatment. Immunocytochemistry demonstrated that expression of Bax was increased and Bcl-2 expression was decreased after ROZ treatment. Conclusion ROZ can inhibit colon cancer HT-29 cells in vitro, which may be associated with its induction of cell apoptosis, up-regulation of Bax expression and down-regulation of Bcl-2 expression. |
| Key words: Rosiglitazone colon cancer proliferation apoptosis |
