| 引用本文： |
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张敬,$2,石红军,李卓权,郭超,汪丽丽.硒抑制镍诱导的人肺成纤维细胞的DNA损伤[J].同济大学学报（医学版）,2004,(3):189-191. [点击复制]
- .The Inhibited Effects of Selenium on DNA Damage Induced by Nickel in Human Lung Fibroblasts[J].同济大学学报（医学版）,2004,(3):189-191. [点击复制]
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| 摘要: |
| 目的 探讨三氧化二镍(Ni2O3)对人肺成纤维细胞(human lung fibroblasts,HLF)的DNA损伤作用以及Na2SeO3的保护作用。方法 应用单细胞凝胶电泳(single cell gel electrophoresis,SCGE)检测DNA损伤。结果 Ni2O3 处理HLF细胞4 h,SCGE检测出DNA损伤程度高于对照组,差异有显著性(P<0.01)。而Ni2O3 Na2SeO3 处理组DNA损伤程度低于Ni2O3 组,差异有显著性(P<0.01)。结论 硒可抑制镍诱导的人肺成纤维细胞的DNA损伤。 |
| 关键词: 亚硒酸钠,三氧化二镍,DNA损伤,单细胞凝胶电泳 |
| DOI： |
| 修订日期：2004-03-20 |
| 基金项目:铁道部科研基金资助项目(J99022) |
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| The Inhibited Effects of Selenium on DNA Damage Induced by Nickel in Human Lung Fibroblasts |
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| Abstract: |
| Objective To study the mechanisms of inhibited effects of Na 2SeO 3 on DNA damage induced by Ni 2O 3 in human lung fibroblasts (HLF) cells.Methods Human lung fibroblasts were cultured in vitro and treated with Ni 2O 3 and Ni 2O 3 Na 2SeO 3,res |
| Key words: Ni 2O 3,Na 2SeO 3,DNA damage,single cell gel,electrophoresi |
