| 引用本文： |
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汪燕芳,赵建阳,倪亚明,成国祥.转基因山羊乳中EPO活性检测[J].同济大学学报（医学版）,2004,(3):187-188191. [点击复制]
- .Detection of Biological Activity of EPO Expressed in Transgenetic Goat Milk[J].同济大学学报（医学版）,2004,(3):187-188191. [点击复制]
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| 摘要: |
| 目的 对转基因山羊乳中 EPO的生物学活性进行检测。方法 用网织红细胞法检测体内生物活性,用ELISA法和 MTT法检测体外生物活性。结果 羊乳中 EPO体内生物学活性明显低于其体外生物学活性。结论推测我们所得的重组EPO的N糖基高分支的比例小及唾液酸化程度偏低,造成其体内生物活性下降。 |
| 关键词: 红细胞生成素,ELISA法,糖蛋白 |
| DOI： |
| 修订日期：2003-12-24 |
| 基金项目: |
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| Detection of Biological Activity of EPO Expressed in Transgenetic Goat Milk |
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| Abstract: |
| Objective To detect biological activity of rhEPO expressed in transgenetic goat milk.Methods The biological activity in vivo of rhEPO was determined by reticulacyte counting method.The biological activity in vitro of rhEPO was determined by ELISA and MT |
| Key words: erythropoietin,ELISA,glycoprotein |
