| 摘要: |
| 目的 阐明胃癌的发生中多种抑癌基因和癌基因的甲基化情况。方法 提取去甲基化制剂5-氮脱氧胞苷(5 aza 2’ deoxycytidine,5 aza dC)干预后的胃癌细胞RNA,RT-PCR方法检测p16INK4A等多种基因的表达情况。结果 5 aza dC干预后两种胃癌细胞系中p16INK4A的表达增强,且不同的胃癌细胞株表达增强最明显时的时间与浓度不同。结论 研究的胃癌细胞系中p16INK4A启动子区的甲基化是它在胃癌细胞系表达减弱的主要原因之一。 |
| 关键词: 胃肿瘤细胞,p16INK4A,抑肿瘤基因,肿瘤基因,人 |
| DOI： |
| 修订日期：2004-02-08 |
| 基金项目:国家自然科学基金资助项目(NO:30170413) |
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| The Expression of Tumor-associated Genes and Methylation in Human Gastric Cancer Cells |
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| Abstract: |
| Objective To investigate the effects on DNA methylation of tumor suppressor gene and oncogenes in human gastric cancer cells.Methods Two gastric cancer cell lines (MKN-45,and HGC-27) were treated with DNA methyltransferase inhibitor,5-aza-2'-deoxycytidi |
| Key words: gastric cancer cell lines,5-aza-dC,DNA methylation,p16 INK4A tumor suppressor gene,oncogene,men |
