| 引用本文： |
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白振东,李培昕,张宁彦,等.miR-592靶向Cep135调控小鼠胚胎干细胞自我更新及分化[J].同济大学学报（医学版）,2019,40(5):542-547，562. [点击复制]
- BAI Zhen-dong,LI Pei-xin,ZHANG Ning-yan,et al.Effects of miR-592 on self-renewal and differentiation of embryonic stem cells by targeting Cep135[J].同济大学学报（医学版）,2019,40(5):542-547，562. [点击复制]
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| 摘要: |
| 目的 探讨miR-592在小鼠胚胎干细胞（mouse embryonic stem cells, mESCs）自我更新及分化过程中的作用。方法 通过转染试剂将miR-592表达质粒转入mESCs，以过表达miR-592。利用qRT-PCR及Western印迹法分析miR-592对mESCs分化的影响。利用TargetScan数据库预测miR-592作用的靶基因，并通过双荧光素酶报告实验验证靶基因。利用Western印迹法探究miR-592及其靶基因对mESCs自我更新的影响。结果 qRT-PCR及Western印迹法实验结果表明miR-592促进mESCs向外胚层方向分化。生物信息学分析及双荧光素酶报告实验的结果表明Cep135是miR-592作用的靶基因，而Western印迹法实验发现Cep135能够促进mESCs的自我更新。结论 miR-592通过靶向下调Cep135的表达，以抑制mESCs的自我更新，进而促使mESCs向外胚层方向分化。 |
| 关键词: miR-592 Cep135 胚胎干细胞 自我更新 分化 |
| DOI：10.16118/j.1008-0392.2019.05.003 |
| 投稿时间：2019-04-01 |
| 基金项目:国家自然科学基金（81771417） |
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| Effects of miR-592 on self-renewal and differentiation of embryonic stem cells by targeting Cep135 |
| BAI Zhen-dong,LI Pei-xin,ZHANG Ning-yan,CHENG Jian,ZHANG Jing,ZHANG Jun |
| (Tongji University School of Medicine, Shanghai 200092, China;Tongji University School of Life Science and Technology, Shanghai 200092, China) |
| Abstract: |
| Objective To investigate the role of miR-592 in the process of self-renewal and differentiation of mouse embryonic stem cells（mESCs）. Methods The miR-592 expression plasmid was transfected into mESCs using a transfection reagent to overexpress miR-592. The effects of miR-592 on the differentiation of embryonic stem cells were analyzed by qRT-PCR and Western blotting. The target gene of miR-592 was predicted with the TargetScan database, and verified by the dual luciferase reporter gene system. The effect of miR-592 and its target genes on self-renewal of mESCs was examined by Western blotting. Results The results of qRT-PCR and Western blotting showed that miR-592 promoted the differentiation of mESCs into the ectodermal direction. The results of bioinformatics and dual luciferase reporter gene systems confirmed that Cep135 was the target gene of miR-592. Moreover, Western blotting results showed that Cep135 promoted the self-renewal of mESCs. Conclusion MiR-592 may inhibit the self-renewal of mESCs by targeting Cep135. Therefore, miR-592 can promote the differentiation of mESCs into the ectodermal direction. |
| Key words: miR-592 Cep135 embryonic stem cells self-renewal differentiation |
