| 引用本文： |
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赵君勇,房林.TBB对乳腺癌细胞miR-411表达及细胞凋亡的影响[J].同济大学学报（医学版）,2017,38(4):31-36. [点击复制]
- ZHAO Jun-yong,FANG Lin.Expression of miR-411 in breast cancer cells with TBB treatment and its relation to cell apoptosis[J].同济大学学报（医学版）,2017,38(4):31-36. [点击复制]
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| 摘要: |
| 目的 观察TBB作用下乳腺癌细胞中miR-411的表达,研究过表达miR-411对乳腺癌细胞凋亡和细胞周期改变的影响。方法 Real-time PCR验证生物芯片检测的差异miRNAs,流式细胞术分析转染前后细胞周期及细胞凋亡情况,针对miR-411进行下游靶基因预测,以real-time PCR验证预测结果。结果 TBB作用下MCF-7细胞株中miR-411显著升高；与对照组相比,转染组早期凋亡细胞比例升高；FoxO1 mRNA表达含量显著增高。结论TBB能够改变乳腺癌MCF-7细胞株中miR-411的表达,过表达miR-411能够抑制乳腺癌细胞株的凋亡,其机制可能与通过影响FoxO1表达有关。 |
| 关键词: 乳腺肿瘤 蛋白激酶CK2的抑制剂 miR-411 细胞凋亡 |
| DOI：10.16118/j.1008-0392.2017.04.007 |
| 投稿时间：2017-04-18 |
| 基金项目: |
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| Expression of miR-411 in breast cancer cells with TBB treatment and its relation to cell apoptosis |
| ZHAO Jun-yong,FANG Lin |
| (Dept. of General Surgery, Tenth People's Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To investigate the expression of miR-411 in breast cancer cells after treated with TBB and its relations to cell cycle and apoptosis. Methods Breast cancer MCF-7 cells were transfected with hsa-miR-411-mimic and treated by 4,5,6,7-tetra-bromo-benzotriazole (TBB). The expression of microRNAs in MCF-7 cells were detected by microRNAs array, and real-time PCR. The cell cycle and apoptosis of MCF-7 cells were analyzed by flow cytometry after hsa-miR-411 mimics transfection. The possible target of miR-411 was forecasted by bioinformatics tools and the expression of the possible target gene was detected by real-time PCR. Results Real-time PCR showed that the expression of miR-411 was changed after TBB treatment in MCF-7 cells. Flow cytometry showed that early apoptotic cells in transfected groups dramatically increased compared to control groups. Real-time PCR showed that the expression of FoxO1 mRNA was significantly upregulated. Conclusion The expression of miR-411 is altered after the introduction of CK2 inhibitor TBB. Transfection with miR-411 mimics can increase phase G0/G1 cells, inhibiting cell proliferation and promoting apoptosis. FoxO1 gene may be involved in the pathway of tumor cell proliferation and apoptosis. |
| Key words: breast cancer TBB miR-411 cells apoptosis |
