| 引用本文： |
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李军亮,吴登龙,黄盛松,等.miRNA-29a调控H3K4甲基化在前列腺癌病理机理中的作用[J].同济大学学报（医学版）,2016,37(6):6-11. [点击复制]
- LI Jun-liang,WU Deng-long,HUANG Sheng-song,et al.miRNA-29a inhibits proliferation and induces apoptosis of prostate cancer cells by regulating H3K4 methylation[J].同济大学学报（医学版）,2016,37(6):6-11. [点击复制]
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| 摘要: |
| 目的 探讨在前列腺癌中miR-29a与H3K4特异性去甲基化酶—KDM5B之间的关系,验证miR-29a通过调控KDM5B的表达来抑制前列腺癌细胞的增殖并诱导其凋亡。方法 将miR-29a模拟物(miR-29a mimic)片段和含野生型KDM5B基因3′-非翻译区的荧光素酶报告载体(KDM5B-wt)共转染至前列腺癌PC3和Lncap细胞后,进行荧光素酶活性检测。采用脂质体转染法将miR-29a mimics转染入人前列腺癌PC3和Lncap细胞中,MTT实验检测细胞增殖情况；流式细胞术检测细胞周期和细胞凋亡；Real-Time PCR和Western印迹法检测KDM5B表达水平。结果 转染miR-29a mimics后,KDM5B的表达被明显抑制 (P<0.01),蛋白表达水平也明显低于NC组。与NC组相比,转染miR-29a mimics组细胞增殖能力显著降低(P<0.01),细胞生长被阻滞在S/G2期,细胞凋亡率较NC组增加(P<0.01)。结论 miR-29a可以通过调控H3K4特异性去甲基化酶-KDM5B的表达而抑制前列腺癌PC3和Lncap细胞的增殖。miR-29a有希望成为诊断和治疗前列腺癌新的分子标记和靶点。 |
| 关键词: 前列腺肿瘤 miRNA-29a KDM5B基因 去甲基化酶 |
| DOI：10.16118/j.1008-0392.2016.06.002 |
| 投稿时间：2015-12-14 |
| 基金项目:国家自然科学基金面上项目(81172426)；上海市教育委员会科研创新项目(12ZZ034) |
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| miRNA-29a inhibits proliferation and induces apoptosis of prostate cancer cells by regulating H3K4 methylation |
| LI Jun-liang,WU Deng-long,HUANG Sheng-song,BIAN Cui-dong,YUAN Tao,GUI Ya-ping |
| (Dept. of Urology, Tongji Hospital, Tongji University, Shanghai 200065, China) |
| Abstract: |
| Objective To investigate the effect of miRNA-29a on cell proliferation and apoptosis of prostate cancer cells and its mechanism. the—KDM5B in prostate cancer, verify miR-29a suppresses prostate cancer (PCa) cell proliferation and induces apoptosis via KDM5B protein regulation. Methods miR-29a mimic and luciferase reporter vector KDM5B-wt were co-transfected into prostate cancer PC3 and Lncap cells, and the luciferase activity was detected. The cell proliferation was examined by MTT assay, cell cycle and apoptosis was assessed by flow cytometry, the mRNA and protein expression levels of KDM5B, a specific demethylase of H3K4, were detected by real-time PCR and Western blotting, respectively. Results After transfection, the relative expression level of KDM5B in miR-29a mimic group was significantly reduced (P<0.01) as compared to that of NC group, and the protein level was also significantly decreased.Compared to NC group, the cell proliferation in miR-29a mimic group was significantly inhibited (P<0.01), the cell cycle was arrested at S/G2 phase, and the cell apoptosis rate was increased (P<0.01). Conclusion miR-29a can suppress the cell proliferation by targeting KDM5B expression, a specific demethylase of H3K4, in prostate cancer PC3 and Lncap cells, which indicates that miR-29a might be used as a new molecular marker and target for diagnosis and treatment of prostate cancer. |
| Key words: prostate cancer miRNA-29a KDM5B gene demethylase |
