| 摘要: |
| 目的 通过建立大鼠心肌缺血再灌注(ischemia/ reperfusion, I/R)模型,探索药物烟酰胺腺嘌呤二核苷酸(NAD+)在大鼠心肌缺血再灌注损伤中的保护作用。方法 采用手术结扎前降支建立大鼠心肌I/R模型,20只Sprague-Dawley ( SD)大鼠随机分入I/R+NAD+组(n=10)和I/R+NS组(n=10),通过血清肌钙蛋白(cTn-I),TTC染色检测心肌损伤程度, TUNEL染色用于检测缺血区域凋亡信号。结果 成功建立大鼠I/R模型,I/R+NAD+组大鼠血清cTn-I水平显著低于I/R+NS组[(10.01±3.26)ng/ml vs. (57.17±14.83)ng/ml, P＜0.01]。TTC染色结果显示: I/R+NAD+组大鼠心脏梗死体积显著低于I/R+NS组[(68.06±16.04)mm3 vs. (11.42±9.65)mm3, p=0.02]。TUNEL染色结果显示: I/R+NAD+组大鼠心脏梗死区域凋亡信号显著低于I/R+NS组。结论 NAD+在大鼠心肌缺血再灌注损伤具有显著保护作用,而其具体保护机制需进一步研究。 |
| 关键词: 缺血再灌注损伤 NAD+ 心肌梗死 大鼠 |
| DOI：10.16118/j.1008-0392.2016.03.006 |
| 投稿时间：2016-01-31 |
| 基金项目:上海交通大学医工交叉研究基金(YG2014MS74)；上海市胸科医院科技发展基金(2014YZDH20300) |
|
| Protective effect of NAD+ in heart ischemia/reperfusion injury |
| ZHANG You-jun,QU Xin-kai |
| (Dept. of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China) |
| Abstract: |
| ObjectiveTo investigate the effect of nicotinamide adenine dinucleotide (NAD+) in protection of myocardium from ischemia/reperfusion injury. Methods The ischemic/reperfusion model was induced with surgical ligation of left anterior descending (LAD) in 20 SD rats. NAD+ 20mg/kg or same volume of NS were given i.v. immediately after LAD ligation to rats in I/R+ NAD+ group (n=10) or I/R +NS group (n=10). Serum cTn-I was measured; TTC staining and TUNEL assay were performed. Results Serum cTn-I level was significantly higher in I/R+NS group than that in I/R+NAD+ group [(57.17±14.83)ng/ml vs. (10.01±3.26)ng/ml, P<0.01]. TTC staining showed that the infarct volume in I/R+NS group was higher than that in I/R+NAD+ group [(68.06±16.04)mm3 vs. (11.42±9.65)mm3, P=0.02]. TUNEL assay showed that high apoptosis signal induced by I/R injury was significantly reduced by NAD+ administration. Conclusion NAD+ can significantly protect the heart from I/R injury. |
| Key words: ischemia/reperfusion injury NAD+ myocardial infarction ret |
