(Dept.of Radiology, Tenth People's Hospital, Tongji University, Shanghai 200072, China;Institute of High Energy Physics, ChineseAcademy of Sciences, Beijing 100049, China;Dept.of Radiology, Tenth People's Hospital, Tongji University, Shanghai 200072, China;Dept.of Radiology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China)
Abstract:
Objective To investigate microstructural changes in chronic glomerulonephritis(CGN) under diffraction enhanced imaging(DEI) technology of synchrotron radiation(SR) in rabbit model. Methods Chronic glomerulonephritis(CGN) was induced by injection of doxorubicin hydrochloride in 5 New Zealand white rabbits. The blood and urine tests and kidney histological examination were performed 2 months after CGN induction. The kidney tissues were fixed into 4% formalin solution for one week before DEI experiment. The experiment was performed at Beijing Synchrotron Radiation Facility(BSRF) 4W1A beam line(beam energy was 14keV). On routine scanning process, the rocking curve was detected, and slope position on the curve was selected for a 360° spatial CT scan, at last the DEI reconstruction software was applied for 3-dimensional image, meanwhile the DEI image grey value between chronic CGN group and control group was analyzed by MATLAB and SPSS. ResultsWithout radio-contrast, DEI provided clear visibility of the microstructural views of artery, vein, straight collecting ducts, papillary tubules, glomeruli of kidneys in both groups, with the spatial resolution of 10μm. The cortex of CGN lost more gray value comparing to the normal one. There was good correlation between DEI images and histopathological images. The microstructural change of CGN kidney was revealed, which was based on the advantage of phase-contrast imaging(PCI) mechanism, and the diagnosis value of CGN by synchrotron radiation(SR) phase-contrast imaging(PCI) technology was evaluated.Conclusion Synchrotron radiation diffraction enhanced imaging(DEI) provides non-invasive and non-contrast diagnosis of CGN in animal model, which may used in clinical diagnosis after further improvement.
