| 引用本文： |
-
韩洋,马雨水,符达,等.TGFβR2作为非小细胞肺癌伴随诊断分子的探讨[J].同济大学学报（医学版）,2015,36(6):19-23. [点击复制]
- HAN Yang,MA Yu-shui,FU Da,et al.TGFβR2 as a bio-maker for companion diagnostics of non-small cell lung cancer[J].同济大学学报（医学版）,2015,36(6):19-23. [点击复制]
|
|
| 摘要: |
| 目的 定量分析转化生长因子受体2(Transforming growth factor receptor 2, TGFβR2)在非小细胞肺癌(non-small cell lung cancer, NSCLC)及其癌旁组织中的表达,探讨TGFβR2的表达在NSCLC伴随诊断(companion diagnostic,CD)中的意义。方法 取患者手术中立即取材置于液氮中的新鲜组织标本(90例NSCLC组织,40例癌旁肺组织),抽提RNA,采用实时荧光定量PCR(RT-qPCR)技术检测标本。TGFβR2基因表达量通过2-ΔΔCT方法计算。TGFβR2的表达用(x±s)呈现。独立样本t检验比较两组差异,应用Kaplan-Meier生存分析来评估总生存期(Overall survival, OS)和无进展生存期(Progression-free survival, PFS)。结果 实验结果表明NSCLC组织中TGFβR2的表达(3.19±4.28)显著高于癌旁肺组织(1.01±0.41),二者差异具有统计学意义。生存分析显示TGFβR2高表达的NSCLC患者预后不良,总生存期和无进展生存期明显降低。结论 TGFβR2可以作为NSCLC潜在的伴随诊断分子标志物。 |
| 关键词: 转化生长因子受体2 非小细胞肺肿瘤 伴随诊断 荧光定量PCR |
| DOI：10.16118/j.1008-0392.2015.06.004 |
| 投稿时间：2015-06-23 |
| 基金项目:国家自然科学基金(81371595) |
|
| TGFβR2 as a bio-maker for companion diagnostics of non-small cell lung cancer |
| HAN Yang,MA Yu-shui,FU Da,CONG Xian-ling,YU Fei,LV Zhong-wei |
| (Dept.of Nuclear Medicine, Tenth People's Hospital, Tongji University, Shanghai 200072, China;Dept.of Dermatology, China Japan Union Hospital, Jilin University, Changchun 130031, China) |
| Abstract: |
| Objective To determine the expression levels of transforming growth factor receptor 2(TGFβR2) in non-small cell lung cancer(NSCLC) and its significance. Methods Total RNA was extracted from NSCLC tissue(n=90) and adjacent non-cancerous tissue(n=40), Real-Time quantitative PCR was performed. The 2-ΔΔCT method was used to quantify the expression levels of TGFβR2. Independent t-test was performed to examine differences between two groups, Kaplan-Meier curves were used to determine overall survival(OS) and progression-free survival(PFS) of patients. ResultsTGFβR2 expression in NSCLC tissue was significantly higher than that in adjacent non-cancerous tissue(3.19±4.28 vs 1.01±0.41,P<0.05). Kaplan-Meier survival analysis demonstrated that prognosis was worse in patients with high expression of TGFβR2. Expression of TGFβR2 was significantly associated with decreased OS and PFS in NSCLC patients. Conclusion TGFβR2 might be used as a bio-marker for companion diagnostics of non-small cell lung cancer. |
| Key words: TGFβR2 NSCLC companion diagnostics RT-qPCR |
