| 引用本文： |
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周静,邱鹏,邢准,等.七氟烷对卵白蛋白致敏的高反应气道平滑肌cAMP的影响[J].同济大学学报（医学版）,2015,36(4):25-28. [点击复制]
- ZHOU Jing,QIU Peng,XING Zhun,et al.Effect of sevflurane on cAMP levels in smooth muscle cells of ovalbumin-sensitized hyperresponsiveness airway in guinea pigs[J].同济大学学报（医学版）,2015,36(4):25-28. [点击复制]
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| 摘要: |
| 目的 观察七氟烷对卵白蛋白致敏的豚鼠的高反应气道平滑肌细胞内环磷酸腺苷(cAMP)的影响。方法40只雄性豚鼠随机分为5组:正常组、致敏组、致敏对照组、致敏2%七氟烷组和致敏4%七氟烷组,每组8只。应用卵白蛋白和测量肺阻力变化曲线建立并评价豚鼠的致敏气道模型。检测致敏豚鼠气道平滑肌细胞cAMP的表达,评价不同浓度七氟烷对高反应致敏气道的扩张作用。结果 与正常组相比,致敏组能显著升高豚鼠的肺阻力变化曲线；与致敏对照组相比,致敏2%七氟烷组和致敏4%七氟烷组的气道平滑肌细胞内cAMP的表达明显增高。结论 七氟烷能够增加卵白蛋致敏的豚鼠的气道平滑肌细胞内cAMP的表达,提示七氟烷可以通过提高高反应致敏气道平滑肌cAMP的表达发挥扩张作用。 |
| 关键词: 七氟烷 环磷酸腺苷 气道平滑肌 高反应气道 豚鼠 |
| DOI：10.16118/j.1008-0392.2015.04.005 |
| 投稿时间：2015-02-01 |
| 基金项目:辽宁省自然科学基金(2014021023) |
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| Effect of sevflurane on cAMP levels in smooth muscle cells of ovalbumin-sensitized hyperresponsiveness airway in guinea pigs |
| ZHOU Jing,QIU Peng,XING Zhun,DONG You-jing |
| (Dept. of Anesthesiology, Shengjing Hospital, China Medical Uneversity, Shenyang 110004, Liaoning Province, China) |
| Abstract: |
| Objective To investigate the effects of sevoflurane on cAMP levels in smooth muscle cells of ovalbumin-sensitized hyperresponsiveness airway in guinea pigs. Methods Forty male guinea pigs were randomly divided into 5 groups:normal group, sensitized group, sensitized control group, sensitized 2% sevoflurane group and sensitized 4% sevoflurane group(n=8 in each group). The lung resisitance was recorded and the dose-response curves for acetylcholine of lung resistance were used to evaluate the sensitized airway model. The intracellular cyclic adenosine monophosphate(cAMP) levels in airway smooth muscle cells were measured to evaluate the brochodilator effect of sevoflurane. ResultsThe dose-response curves for acetylcholine of lung resistance were elevated significantly in the sensitized group compared to normal group. The intracellular cAMP levels in the airway smooth muscle cells were higher in sensitized 2% sevoflurane and sensitized 4% sevoflurane groups than those in sensitized control group. Conclusion Sevoflurane can increase the intracellular cAMP levels in airway smooth muscle cells of sensitized guinea pigs, which suggests that sevoflurane has a brochodilator effect in sensitized airways. |
| Key words: sevoflurane cAMP airway smooth muscle hyperresponsiveness airway guinea pigs |
