| 引用本文： |
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祝国富,林莹,李伟明.Vaspin对巨噬细胞THP-1向泡沫细胞转变的抑制作用[J].同济大学学报（医学版）,2015,36(3):31-34, 47. [点击复制]
- ZHU Guo-fu,LIN Ying,LI Wei-ming.Inhibitory effects of vaspin on bubbling of macrophages THP-1 in vitro[J].同济大学学报（医学版）,2015,36(3):31-34, 47. [点击复制]
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| 摘要: |
| 目的 腹腔脂肪型丝氨酸蛋白酶抑制剂(visceral adipose tissue-derived serine protease inhibitor, vaspin)是新发现的脂肪因子,对代谢性疾病具有改善作用。该研究旨在研究vaspin对体外培养的THP-1细胞泡沫化的影响。方法 100nM佛波酯孵育THP-1巨噬细胞48h后分为对照组,氧化低密度脂蛋白组(ox-LDL浓度50μg/ml,48h),vaspin干预组(vaspin终浓度100ng/ml,预孵24h)；采用油红0染色法检测vaspin对THP-1细胞泡沫化的影响;Real-time PCR和Western blot法检测vaspin对THP-1细胞中酰基辅酶A胆固醇酰基转移酶-1(acy1-coenzyme A:cholesterol acyltrasferase-1,ACAT-1)、ATP结合盒转运体A1(ATP binding casstte transporterA1,ABCA1)及细胞清道夫受体-A1(scavenger receptor-A1, SR-A1)mRNA及蛋白表达水平的影响。结果 与ox-LDL组比较,ox-LDL+vaspin组巨噬细胞泡沫化程度明显下降(P＜0.05);ox-LDL+vaspin组ACAT-1和SR-A1 mRNA及蛋白表达水平明显降低(P＜0.05),而ABCA1 mRNA及蛋白表达水平明显升高(P＜0.05)。结论 vaspin能够抑制巨噬细胞向泡沫细胞的转化,发挥抗动脉粥样硬化(atherosclerosis, As)的作用。 |
| 关键词: 腹腔脂肪型丝氨酸蛋白酶抑制剂 巨噬细胞 泡沫细胞 酰基辅酶A胆固醇酰基转移酶-1 ATP结合盒转运体A1 细胞清道夫受体-A1 |
| DOI：10.16118/j.1008-0392.2015.03.007 |
| 投稿时间：2015-03-17 |
| 基金项目: |
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| Inhibitory effects of vaspin on bubbling of macrophages THP-1 in vitro |
| ZHU Guo-fu,LIN Ying,LI Wei-ming |
| (Dept. of Cardiology, Tenth People's Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To investigate the effect of vaspin on bubbling of macrophages THP-1 in vitro. Methods Human THP-1 macrophages were induced by phorbol-12-myristate 13-acetate(PMA,100nmol/L) for 48h,then cells were divided into three groups:control group, ox-LDL group(at a final concentration of 50μg/ml for 48h), ox-LDL+vaspin group(vaspin at a final concentration of 100ng/ml, pretreated for 24h). The effect of vaspin on bubbling of THP-1 cells was analyzed by Oil Red 0 staining. The expressions of mRNAs and proteins of ACAT-1,ABCA1 and SR-A1 were detected by real-time PCR and Western blotting, respectively. Results As compared with ox-LDL group, the bubbling level in ox-LDL+vaspin group was decreased significantly. The mRNA and protein expression levels of ACAT-1 and SR-A1 in ox-LDL+vaspin group significantly decreased, while those of ABCA1 mRNA and protein increased significantly(P＜0.05). Conclusion Vaspin inhibits the transformation of macrophages to foam cells, thus playing an anti-atherosclerosis role. |
| Key words: vaspin macrophages foam cells acy1-coenzyme ATP binding cassette transporter A1 acyl-coenzyme A:cholesterolacyltrasferase-1(ACAT-1) scavenger receptor-A1(SR-A1) |
