| 引用本文： |
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李凤,翁文浩,龙吟,等.RNA解旋酶DDX39对肾透明细胞癌增殖的影响[J].同济大学学报（医学版）,2014,35(5):11-15. [点击复制]
- li Feng,WENG Wen-hao1,LONG Yin,et al.Effect of RNA helicase DDX39 on proliferation of renal cancer 786-0 cells[J].同济大学学报（医学版）,2014,35(5):11-15. [点击复制]
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| 摘要: |
| 目的 通过小干扰RNA沉默检测DZJX39基因对肾透明细胞癌增殖的影响及其相关机制。方法应用 脂质体转染法将DDX39-siRNA转染人肾透明细胞癌786-0细胞,试验设空白对照组、阴性对照组、小干扰RNA转 染组(￡>DX39-siRNA)。应用实时荧光定量PCR和Western印迹法检测￡>DX39-siRNA的干扰效率;MTT法和平板 克隆形成实验检测DDX39对786-0细胞增殖的影响；Western印迹法检测细胞增殖指标PCNA;流式细胞术检测 D￡)A39-siRNA干扰后786-0细胞周期的变化。结果与阴性对照组相比，转染DDX39-siRNA后，786-0细胞中 DDX39 mRNA及其蛋白的表达水平明显下降,差异有统计学意义（尸<0. 05); Z)DX39-siRNA干扰后"786-0细胞增 殖能力受到抑制，克隆形成能力明显下降;s期细胞减少,G2/M期细胞百分比明显增加（P <0.05)。结论DDX39 敲减后可显著抑制肾癌细胞786-0细胞增殖,并阻滞细胞于G2/M期。 |
| 关键词: M?9基因 细胞增殖 端粒 RNA解旋酶 |
| DOI：10.3969/j. issnl008-0392.2014.05.003 |
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| 基金项目:基金项目：国家自然科学基金(81171883) |
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| Effect of RNA helicase DDX39 on proliferation of renal cancer 786-0 cells |
| li Feng,WENG Wen-hao1,LONG Yin,li Zhi |
| (Dept, of Clinical Laboratory, Tenth People’s Hospital, Tongji University, Shanghai 200072, China;Dept, of Clinical Laboratory, Yangpu Hospital, Tongji University, Shanghai 200090, China) |
| Abstract: |
| Objective To investigate the effect of DDX39 on human renal carcinoma 786-0 cells by siRNA silence. Methods The DDX39-siRNA targeting human DDX39 gene was transfected into 786-0 cells by SuperFectin? II in vitro siRNA transfection reagent. The cells were divided into blank control group, negative control group and siRNA interference group (Z>Z)X39-siRNA). The expression of DDX39 mRNA and protein was detected by Real-Time PCR and Western blotting, respectively. The cell proliferation was measured by MTT method and colony formation. The proliferation protein marker was detected by Western blotting and the cell cycle was detected by flow cytometry. Results As compared with the negative control, the expression level of DDX39 mRNA and protein level was decreased after transfection of Z>Z)X39-siRNA (P <0.05). The proliferation of 786-0 cells was declined and the ability of colony-formation was decreased. The cell cycle of 786-0 cells was arrested at G2/M phase and S phase was decreased (尸 < 0_ 05 ). Conclusion DD^39-siRNA can downregulate expression levels of DDX39 mRNA and protein in human renal cancer 786-0 cells, resulting in the inhibition of cell proliferation and cell cycle. |
| Key words: DDX39 gene cell proliferation telomere RNA helicase |
