| 引用本文： |
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俞蕾,马纪,王勤婉,等.基因Wip1低表达与椎间盘退行性病变的关系[J].同济大学学报（医学版）,2013,34(2):6-10. [点击复制]
- YU Lei,MA Ji,WANG Qin-wan,et al.Low expression of Wipl is related to intervertebral disc degeneration[J].同济大学学报（医学版）,2013,34(2):6-10. [点击复制]
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| 摘要: |
| 目的探讨基因Wipl异常表达与椎间盘退行性疾病（intervertebraldiscdegeneration，IDD）的关系。方法选取62例患者退行性病变的椎间盘组织，通过实时定量PCR测定Wipl基因表达量；siRNA干扰人髓核（nucleuspulposus，NP）细胞Wipl的表达后，通过SA-β-gal染色检测细胞衰老情况；通过MTT检测NP细胞的增殖能力；通过实时定量PCR检测NP细胞衰老分子标记的表达水平；γ射线照射siRNA转染后的NPP3细胞，分别于不同时间点行SA-β-gal染色检查。结果62例病变椎间盘组织中，18例Wipl基因的表达明显下降（P＜0.01）；siRNA干扰人NP细胞Wipl表达后，衰老细胞数量明显增加；MTT结果表明，干扰Wipl的表达能够显著抑制人NP细胞的增殖；P3细胞培养8d，实时定量PCR检测发现，Col2al、Agc、Ver的表达水平显著下调，MMP3的表达水平明显增高。γ射线照射NP细胞后，SA-β-gal染色显示，Wipl表达组出现明显的衰老现象。结论Wipl的低表达与椎间盘退行性病变的发生相关，干扰Wipl的表达能够促进NP细胞衰老，而过表达Wipl可以有效抑制细胞衰老的发生。 |
| 关键词: 椎间盘退行性疾病 DNA损伤修复反应 细胞衰老 |
| DOI：10.3969/j.issn1008-0392.2013.02.002 |
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| 基金项目:国家自然科学基金（81171778）;国家自然科学基金（30971466）;国家自然科学基金（31171086） |
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| Low expression of Wipl is related to intervertebral disc degeneration |
| YU Lei1,MA Ji2,WANG Qin-wan2,YU Yong-chun2,PAN Qiu-hui2,LI Jing-hua2 |
| (1. Dept. of Clinical Laboratory, Tenth People＇s Hospital, Tongji University, Shanghai 200072, China;2. Dept. of Central Laboratory, Tenth People＇s Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To investigate the association between aberrant expression of Wipl and intervertebral disc degeneration （IDD）. Methods The expression of Wipl was examined by qRT- PCR in 62 discopathy tissue samples. Cell senescence and proliferation was evaluated by SA-13-gal and MTT when Wipl was knocked down by siRNA interference of in nucleus pulposus （NP） cells. Senescence marker expression was detected by qRT-PCR. The NP cells treated by Wipl siRNA were exposed to γ radiation and then evaluated for cell senescence by SA-β-gal at different time points. Results The expression of Wipl gene was down-regulated in 18/62 discopathy tissue samples compared with normal tissues. Moreover, the depletion of Wipl by RNA interference （RNAi） significantly increased NP cell senescence. MTT analysis showed that knockdown of Wipl markedly inhibited the proliferation of NP cells. In addition, qRT-PCR data indicated that the expression ofCol2al, Agc and Ver were down-regulated, while MMP3 was up-regulated after 8 days of P3 cell cultured. SA-[3-gal staining showed that expression of Wipl induced NP cell senescence significantly when cells exposed to y radiation. Conclusion The aberrant expression of Wipl is associated with discopathy. Knockdown of Wipl may increase senescence of NP cells, while overexpression of Wipl may inhibit pathogenesis of discoDathv. |
| Key words: intervertebral disc degeneration DNA damage repair cell senescence |
