| 引用本文： |
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刘宇,盛敏杰,林安娟,等.PPARγ配体盐酸吡格列酮对MRL/Ipr小鼠泪腺炎症干预的研究[J].同济大学学报（医学版）,2013,34(1):8-12. [点击复制]
- LIU Yu,SHENG Min-jie,LIN An-juan,et al.Effect of hydrochloride pioglitazone on inflammation of lacrimal gland in MRL/lpr mice[J].同济大学学报（医学版）,2013,34(1):8-12. [点击复制]
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| 摘要: |
| 目的研究过氧化物酶增殖体激活受体γ（peroxisorne proliferator activated receptor γ，PPARγ）激动荆盐酸吡格列酮对干眼模型MRL/lpr小鼠泪腺的影响。方法12周龄MRL/lpr及BLAB／C雌性小鼠各30只，随机分为空白对照组、空白干预组、干眼对照组、干眼干预组。对空白干预组及干眼干预组行盐酸吡格列酮溶液灌胃干预（60mg/kg）。8周后酚红棉线实验检测泪液分泌情况；取各组小鼠泪腺组织行H-E染色，分析淋巴细胞浸润情况；免疫组织化学法及Western印迹法检测泪腺组织炎症因子TNF-α、PPARγ的IL-1β表达情况。结果干眼干预组小鼠泪液分泌量显著大于其他各组（P＜0.05），淋巴细胞浸润情况明显少于干眼对照组，泪腺炎症因子TNF-α和IL-1β表达明显低于干眼对照组，PPARγ表达显著高于干眼对照组（P＜0.05）。结论对MRL/1pr干眼模型小鼠进行盐酸吡格列酮溶液灌胃干预后，可激活PPARγ并使其上调，抑制炎症因子TNF-α及IL-11β的表达，从而干预干眼炎症的进程，改善干眼动物体征及泪腺损害。 |
| 关键词: 干眼 盐酸吡格列酮 过氧化物酶增殖体 MRL lpr 小鼠 |
| DOI：10.3969/j.issn1008-0392.2013.01.002 |
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| 基金项目:上海市自然科学基金(11ZR1427900);上海市卫生局青年科研项目(2010Y164) |
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| Effect of hydrochloride pioglitazone on inflammation of lacrimal gland in MRL/lpr mice |
| LIU Yu,SHENG Min-jie,LIN An-juan,WANG Wei-fang,CHEN Yi-hui |
| (Dept.of Ophthalmology,Tenth People’s Hospital,Tongji University,Shanghai 200072,China) |
| Abstract: |
| Objective To investigate the effect of proxisome proliferators-activated receptor -γ （PPARγ） ligand hydrochloride pioglitazone on the lacrimal gland of MRL/lpr mice. Methods Female MRL/lpr mice （dry eye model） and BLAB/C mice （control） of 12 weeks were randomly divided into 4 groups： dry eye group, dry eye ＋ pioglitazone treatment group, control group and control ＋ pioglitazone group. Mice in groups dry eye ＋ pioglitazone treatment group and control ＋ pioglitazone group were treated with hydrochloride pioglitazone 60 mg/kg daily by oral gavage. Cotton thread test performed 8 weeks after treatment. H-E analyze the invasion of lymphocytes. Expressions interleukin-1β （IL-1β） in lacrimal gland were staining was carried out for lacrimal gland tissues to of PPARγ, tumor necrosis factor-a （TNF-a） and examined by immunohistochemistry staining and Western blotting. Results Tear production was mcreasect mtary eye ＋ pioglitzaone treatment group than that in other groups （P＜0.05 ）. H-E staining showed less invaded lymphocytes in dry eye ＋ pioglitazone treatment group compared with dry eye group. Immunohistochemistry staining showed that the expression of inflammatory factors TNF-a and 1L-1 β was decreased in dry eye ＋ pioglitazone treatment group. Western blotting revealed that the expression of PPARγ protein was increased and TNF-a and IL-1 β was decreased in dry eye ＋ pioglitazone treatment group compared to dry eye group. Conclusion Hydrochloride pioglitazone can intervene the progress of dry eye, which may be associated with up-regulating PPARγ expression and down-regulation TNF-a and IL-1 β expression. |
| Key words: dry eye hydrochloride pioglitazone peroxisome proliferator MRL/lpr mice |
