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孙慧,王倩,张之芬,等.成人急性B淋巴细胞白血病免疫表型分析[J].同济大学学报（医学版）,2012,33(5):62-65. [点击复制]
- SUN Hui,WANG Qian,ZHANG Zhi-fen,et al.Analysis of immunophenotype of B lineage acute lymphoblastic leukemia in adult patients[J].同济大学学报（医学版）,2012,33(5):62-65. [点击复制]
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| 成人急性B淋巴细胞白血病免疫表型分析 |
| 孙慧1,王倩1,张之芬1,武焕玲1,田文君1,刘斌2 |
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| (1. 山东大学附属省立医院检验科,山东 济南,250021;2. 山东大学附属省立医院中心实验室,山东 济南,250021) |
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| 摘要: |
| 目的研究成人急性B淋巴细胞白血病(B cell acute lymphoblastic leukemia,B-ALL)的免疫表型特点。方法采用四色流式细胞术CD45/SSC双参数散点图设门检测145例B-ALL患者免疫表型,并分析白血病相关免疫表型(leukemia associated immunophenotypes,LAIP)。结果所有检测病例中,表达率最高的淋系抗原为CD19、HLA-DR和CD79a,其次为CD34、CD10和CD20;髓系抗原CD13、CD33和CD15常伴随表达,CD117与T系抗原CD7和CD56很少表达。成人不同年龄组中Ⅱ型(CD10+/CD34+型)患者比例最高。B-ALL患者Ⅰ型和Ⅱ型的LAIP检出率较高,分别为100%、90.1%,Ⅲ型和Ⅳ型患者LAIP检出率明显低于Ⅰ型和Ⅱ型,分别为72.7%、63.6%。不同年龄组中,B-ALL各亚型之间及与总体之间检出率差异无统计学意义(P>0.05)。结论流式细胞术分析B-ALL免疫表型对白血病的正确诊断及其微小残留病(minimal residual disease,MRD)检测有重要意义。 |
| 关键词: 急性B淋巴细胞白血病 白血病相关免疫表型 流式细胞术 |
| DOI：10.3969/j.issn1008 -0392.2012.05.014 |
| 投稿时间：2011-10-27 |
| 基金项目:山东省优秀中青年科学家科研奖励基金（BS2012YY025) |
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| Analysis of immunophenotype of B lineage acute lymphoblastic leukemia in adult patients |
| SUN Hui1,WANG Qian1,ZHANG Zhi-fen1,WU Huan-ling1,TIAN Wen-jun1,LIU Bin2 |
| (1.Dept.of Clinical Laboratory,Shandong Provincial Hospital,Shandong University,Jinan 250021,Shandong Province,China;2.Dept.of Central Laboratory,Shandong Provincial Hospital,Shandong University,Jinan 250021,Shandong Province,China)) |
| Abstract: |
| Objective To investigate the immunophenotype of B cell acute lymphoblastic leukemia in adult patients. Methods Immunophenotyping was performed by flow cytometry in 145 adult patients with B cell acute lymphoblastic leukemia. Results The highest incidence of lymphoid associated antigens were CD19, HLA-DR and CD79a, followed by CD34, CD10 and CD20. B-ALL had a higher expression rate of myeloid antigens CD13, CD33 and CD15, CD117 and T antigens (CD7 and CD56) were rarely expressed. Subtype n (CD10 + /CD34 + ) was predominant in three age groups of adults. The LAIP was observed in 100. 0% and 90.1% patients of subtype I and subtype n，respectively, whereas only 72. 7% and 63. 6% in subtype M and subtype IV. There was no significant difference in incidence of LAIP between three age groups (P >0.05) . Conclusion The immunophenotype by flow cytometry is of great value in correct diagnosis and minimal residual disease detection of B cell acute lymphoblasticleukemia. |
| Key words: B cell acute lymphoblastic leukemia leukemia associated immunophenotypes flowcytometry |