| 摘要: |
| 目的 建立离体兔的缺血性心房颤动模型。方法 40只健康雄性的新西兰兔(2.2~2.5 kg),随机分为正常流量灌流组、正常流量灌流+短促高频电刺激组、低灌流模拟心肌缺血组、低灌流模拟心肌缺血+短促高频电刺激组,每组10只。采用Langendorff灌流装置行主动脉逆行灌流,通过缺血和短促高频电刺激诱发心房颤动。结果 正常流量灌流组、正常流量灌流+短促高频电刺激、低灌流模拟心肌缺血组心房颤动的发生率为0%,而低灌流模拟心肌缺血+短促高频电刺激组心房颤动的发生率为90%。结论 通过Langendorff心脏灌流中模拟心肌缺血,并给予短促高频电刺激模拟应激事件,可以稳定地诱导心房颤动的发生。 |
| 关键词: 心房颤动 缺血 新西兰兔 |
| DOI：10.3969/j.issn1008-0392.2012.04.005 |
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| 基金项目:国家自然科学基金（81170224)；上海市自然科学基金（10zr1433000) |
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| Establishment of ischemia-induced atrial fibrillation model in isolated rabbit heart |
| CHEN Xiao-li |
| () |
| Abstract: |
| Objective To establish an ischemia-induced atrial fibrillation (AF) model in the isolated rabbit heart. Methods Forty healthy male New Zealand white rabbits (2.2~2.5 kg) were used in experiments. The isolated heart were randomly assigned to normal perfusion, normal perfusion with burst high-frequency electric stimulation, low perfusion, and low perfusion with burst high -frequency electric stimulation with 10 in each group. The isolated hearts were perfused with Langendorff perfusion system. Results The incidence of AF in group with burst electric stimulation during low perfusion was 90% ； while no AF was induced in other three groups. Conclusion Atrial fibrillation can be stably induced by ischemia with burst electric stimulation in isolated rabbit heart. |
| Key words: atrial fibrillation ischemia New Zealand rabbit |
