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王锋,沈佳庆,王兴鹏.三硝基苯磺酸诱导的慢性胰腺炎模型在疼痛研究中的应用[J].同济大学学报（医学版）,2012,33(4):19-22. [点击复制]
WANG Feng,SHEN Jia-qing,WANG Xing-peng.Application of trinitrobenze sulfonic acid-induced chronic pancreatitis rat model in pain study[J].同济大学学报（医学版）,2012,33(4):19-22. [点击复制]

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三硝基苯磺酸诱导的慢性胰腺炎模型在疼痛研究中的应用
王锋¹,沈佳庆²,王兴鹏^1,3
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摘要:

目的探讨三硝基苯磺酸(trinitrobenze sulfonic acid,TNBS)建立的慢性胰腺炎(chronic pancreatitis,CP)模型在CP腹痛研究中的适用性。方法 2%TNBS逆行胆胰管灌注建立大鼠CP模型。观察血清淀粉酶、脂肪酶和组织学以评价造模是否成功。Von Frey Filament测试检测不同时间点(1、2、3和4周)大鼠腹部的阳性反应,进而推测大鼠胰腺疼痛的改变。结果 TNBS处理4周后,大鼠胰腺出现实质损伤和进行性纤维化,具有CP的组织病理学改变。Von Frey Filament检测显示CP造模后,随着时间推移,大鼠腹部阳性反应逐渐增加。结论 TNBS不但适用于CP动物模型的建立,而且还适用于CP疼痛的基础研究。

关键词: 慢性胰腺炎三硝基苯磺酸动物模型疼痛

DOI：10.3969/j.issn1008-0392.2012.04.004

基金项目:国家自然科学基金（30971359)

Application of trinitrobenze sulfonic acid-induced chronic pancreatitis rat model in pain study

WANG Feng¹,SHEN Jia-qing²,WANG Xing-peng^1,2

()

Abstract:

Objective To evaluate the application of chronic pancreatitis (CP) model induced by trinitrobenze sulfonic acid (TNBS) in pain study. Methods CP was induced by intraductual infusion of 2% TNBS in rats. Histopathological examination and serum amylase and lipase measurement were performed for the validation of CP. Pain resulting from CP was studied as abdominal sensitivity to Von Frey Filament stimulation on week 1，2，3 and 4 after the induction of CP. Results Progressive fibrosis and parenchyma injury were observed in tissue sections after 4 weeks of TNBS treatment. Abdominal pain was observed in TNBS treated rats and the severity was increased over time. Conclusion The rat CP model induced by TNBS is suitable in pain study.

Key words: chronic pancreatitis trinitrobenze sulfonic acid animal model pain