| 摘要: |
| 羊水中存在多能性干细胞,它们不仅阳性表达胚胎干细胞(embryonic stem cell,ESC)表面标志物,如SSEA-4,保持未分化状态的转录因子OCT-4,胚胎多能性维持因子Nanog等;而且阳性表达成人干细胞(adult stem cell,ASC)表面标志物CD29和CD44等,在特定的诱导条件下能够分化为代表三胚层生殖层系。 |
| 关键词: 羊水 多能性干细胞 培养 分化 |
| DOI：10.3969/j.issn1008-0392.2012.01.025 |
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| 基金项目:上海市自然科学基金（10ZR1423500);上海市卫生局科研项目（2008008) |
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| Culture and differentiation of pluripotent stem cells from amniotic fluid |
| LUO Cheng-feng,ZHOU Qian |
| (Dept.of Central Laboratory,Shanghai First Maternity and Infant Hospital,Tongji University,Shanghaii 200040,China) |
| Abstract: |
| Human amniotic fluid contains pluripotent stem cells,which positively express markers of embryonic stem cells,like SSEA-4,Oct-4 and Nanog;and also markers of adult stem cells,like CD29 and CD44.Under specific inducing conditions,they are capable of differentiating into lineages representing three embryonic germ layers. |
| Key words: amniotic fluid pluripotent stem cells culture differentiation |
