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徐铮,李冠武,汤光宇,等.显微CT对兔骨质疏松模型的实验研究[J].同济大学学报（医学版）,2011,32(6):34-38. [点击复制]
XU Zheng,LI Guan-wu,TANG Guang-yu,et al.Experimental study on a rabbit osteoporosis model with micro-CT[J].同济大学学报（医学版）,2011,32(6):34-38. [点击复制]

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显微CT对兔骨质疏松模型的实验研究
徐铮^1,2,李冠武¹,汤光宇¹,刘勇¹,张晓¹
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(1. 同济大学附属第十人民医院影像科,上海200072;2.上海市闵行区莘庄社区卫生服务中心,上海201199)

摘要:

目的探讨去势法联合皮质激素快速诱导兔骨质疏松模型椎体骨小梁Micro-CT动态变化规律。方法 40只8个月龄雌性大白兔随机分为假手术组（A组,n=20）及去势＋肌注甲强龙组（B组,n=20）。术后第2、4、8、10周行血清骨转化标志物检测及离体腰5椎体Micro-CT扫描。每组在每个时间点动物数5只。结果两组vBMD在术后第8周开始,差异有统计学意义,而BVF、Tb.N、Conn.D、Tb.Sp、SMI及Tb.Th分别在术后4、8周开始,差异有统计学意义。B组术后TRAP-5b迅速增高,至第4周达峰值,术后第4、8、10周,两组TRAP-5b差异有统计学意义;而BALP缓慢逐渐增高,至第8周才达峰值,术后第8、10周,两组BALP差异有统计学意义。结论去卵巢＋肌注甲强龙可在8周内快速建立兔OP模型,骨微结构衰败早于vBMD变化而与骨转化标志物同步。

关键词: 显微CT 骨转化标记物骨质疏松骨微结构

DOI：10.3969/j.issn1008-0392.2011.06.007

基金项目:国家自然科学基金(81071134)

Experimental study on a rabbit osteoporosis model with micro-CT

XU Zheng^1,2,LI Guan-wu¹,TANG Guang-yu¹,LIU Yong¹,ZHANG Xiao¹

(1.Dept.of Radiology,Tenth People’s Hospital,Tongji University,Shanghai 200072,China; 2.Community Healty Center of Xinzhuang,Minhang District,Shanghai 201199,China)

Abstract:

Objective To observe the morphological characteristics of osteoporosis(OP) in a overiectomized rabbit model with micro-computed tomography(micro-CT).Methods Forty 8-month-old, skeletally mature,female New Zealand white rabbits were randomly allocated into two groups; group B(n = 20) underwent bilateral overiectomy(OVX) combined with intramuscular injection of methylprednisolone hemisuccinate(MPH);group A(n =20) were used as sham operation controls. Five rabbits from both groups were sacrificed at 2,4,8 and 10 weeks after OVX,respectively.The serum bone metabolism markers were measured and micro-CT scanning of L5 vertebral body was performed.Results The bone mineral density(BMD) of group B at 8 and 10 weeks after OVX was significantly decreased compared to that of group A(P＜0.01).The microstructural parameters BVF, Conn.D,Tb.N,SMI,Tb.Sp and Tb.Th in group B were significantly lower than those in group A at 4 and 8 weeks after OVX.Serum TRAP-5b in group B was significantly increased at 4,8 and 10 weeks after OVX compared to group A(P＜0.01);while serum BALP was significantly increased at 8 and 10 weeks(P＜0.01).Conclusion Osteoporosis can be induced in rabbits by combining overiectomy with corticosteroid administration in 8 weeks.The deterioration of trabecular microarchitecture occurs earlier than that of vBMD,but in synchrony with changes of bone turnover markers.

Key words: micro-CT bone turnover markers osteoporosis bone microstructure