| 引用本文： |
-
徐红梅,沈志超.医学论文中转化、转导、转染和感染的辨析[J].同济大学学报（医学版）,2011,32(3):96-100. [点击复制]
- XU Hong-mei,SHEN Zhi-chao.Distinguish transformation, transduction, transfection and infection in medical papers[J].同济大学学报（医学版）,2011,32(3):96-100. [点击复制]
|
|
| 摘要: |
| 转化、转导、转染和感染是分子生物学实验中基因转移相关的名词，在医学论文中经常使用。它们词形相近、词意容易混淆，医学论文中时常用错。“转化”指通过含外源基因的重组质粒载体将外源基因直接导入原核细胞（如细菌）；“转导”指通过重组病毒载体将外源基因导入真核细胞或原核细胞；“转染”指重组质粒载体或游离核苷酸在脂质体等介导下进入真核细胞；“感染”在基因转移实验中强调重组病毒载体入侵受体细胞的过程。在使用这4个名词时，应仔细分析基因转移实验的4要素——转移物、载体、介导方法、受体细胞类型，而正确区分载体和受体细胞类型是辨析的关键点。当载体是重组质粒时，如受体细胞是原核细胞应使用“转化”，如受体细胞是真核细胞则使用“转染”；当载体是重组病毒时，如强调转移物进入受体细胞应使用“转导”，如强调重组病毒我体进入受体细胞的过程则使用“感染”。 |
| 关键词: 转化 转导 转染 感染 基因转移 医学论文 |
| DOI：10.3969/j.issn1008-0392.2011.03.024 |
|
| 基金项目: |
|
| Distinguish transformation, transduction, transfection and infection in medical papers |
| XU Hong-mei,SHEN Zhi-chao |
| (Dept.of Immunology,Second Military Medical University & Editorial Office of Chinese Journal of Cancer Biotherapy,Shanghai 200433,China) |
| Abstract: |
| Transformation,transduction,transfection and infection are gene transfer related terms in molecular biology experiments,and are widely used in medical papers.As they have similar word forms and meanings,they are often used incorrectly or imprecisely in medical papers.Transformation means exogenous genes entering into prokaryotic cells(such as bacteria) via recombinant plasmid vector containing the genes;transduction means exogenous genes entering into eukaryotic and prokaryotic cells via recombinant virus vector;transfection means recombinant plasmid vector or free nucleic acids entering into eukaryotic cells via lipofectamine or other methods;while infection emphasizes the process that recombinant virus vector enters into receptor cells.In exogenous gene transfer experiments,these four terms can be distinguished through four elements-target molecule, vector,transfer method,and receptor cell,among them distinction of the vector types and receptor cells is the key point.In the case of recombinant plasmid vector,if receptor cells are prokaryotic cells, transfection will be used;if receptor cells are eukaryotic cells,transformation will be used.In the case of recombinant virus vector,transduction emphasizes the result of recombinant virus vector entering into receptor cells,whereas infection emphasizes the entering process of recombinant virus vector into receptor cells. |
| Key words: transformation transduction transfection infection gene transfer medical paper |
