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李骏飞,周大兴,洪先业,等.人参皂苷Rg1、Rb1及Re对人皮肤胶原代谢作用的研究[J].同济大学学报（医学版）,2011,32(3):28-32. [点击复制]
LI Jun-fei,ZHOU Da-xing,HONG Xian-ye,et al.Effects of ginsenosides Rg1, Rb1 and Re on human skin collagen metabolism[J].同济大学学报（医学版）,2011,32(3):28-32. [点击复制]

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人参皂苷Rg1、Rb1及Re对人皮肤胶原代谢作用的研究
李骏飞¹,周大兴¹,洪先业¹,蔡苗²
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(1. 浙江省中医药大学药学院,杭州310053;2. 浙江大学医学院附属第二医院神经科,杭州310009)

摘要:

目的以人成纤维细胞为载体对三种皂苷Rg1、Rb1、Re进行研究。方法 MTT法测定三种药物在不同浓度下对人成纤维细胞增殖的影响，消化法检测羟脯氨酸含量。ELISA法测定细胞分泌I型前胶原、基质金属蛋白酶-1（matrix metalloproteinase-1，MMP-1）、基质金属蛋白酶抑制酶-1（tissue inhibitor of metalloproteinase-1，TIMP-1）的蛋白表达情况，RT—PCR法观察上述三种蛋白的mRNA表达水平。结果与空白组比较，三种单体均能显著提高细胞增殖水平，羟辅氨酸含量、I型前胶原的蛋白质及mRNA表达量，显著降低MMP—1蛋白质及mRNA表达量。结论人参皂苷Rg1、Re、Rb1通过影响成纤维细胞的基因及蛋白质的表达，均显著促进胶原合成、抑制胶原降解，从而提高胶原蛋白总量，改善胶原代谢。

关键词: 人参皂苷胶原I型前胶原基质金属蛋白酶1

DOI：10.3969/j.issn1008-0392.2011.03.007

基金项目:

Effects of ginsenosides Rg1, Rb1 and Re on human skin collagen metabolism

LI Jun-fei¹,ZHOU Da-xing¹,HONG Xian-ye¹,CAI Miao²

(1.Zhejiang Chinese Medical University College of Pharmaceutical Sciences,Hangzhou,310053;2.Dept.of Neurology,Second Affiliated Hospital,School of Medicine, Zhejiang University,Hangzhou,310009)

Abstract:

Objective Ginsenosid plays an important role in anti-aging of skin cells.The effects of ginsenoside monomers on collagen metabolism and its mechanism,however,have not been reported.To investigat the effect of ginsenoides Rg1,Rb1 and Re on human skin collagen metabolism and its mechanism.Methods The proliferation of fibroblasts treated by different concentrations of three ginsenoides was measured with MTT method.The content of hydroxyproline was detected by digestive assay.The expressions of procollagenⅠ,MMP-1 and TIMP-1 proteins and mRNA were measured by ELISA and RT-PCR respectively. Results Compared with the control group,the treatment of three ginsenosides significantly enhanced proliferation of fibroblasts,increased the amount of hydroxyproline,and expression of both protein and mRNA of procollagenⅠand TIMP-1,but significantly decreased expression of MMP-1 protein and mRNA.Conclusion Ginsenosides,Rg1,Re and Rb1 can promote synthesis and suppress degradation of collagen in skin fibroblasts,which may be associated with regulating the mRNA and protein levels of procollagenⅠ,MMP-1 and TIMP-1.

Key words: ginsenoside collagen typeⅠ procollagen matrix metalloproteinase-1