| 引用本文： |
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乐威,钱君海,赵华颖,,等.快速检测精子性染色体分析技术的建立[J].同济大学学报（医学版）,2011,32(1):24-27. [点击复制]
- LE Wei,QIAN Jun-hai,ZHAO Hua-ying,et al.Establishment of a rapid technical assessment of sex chromosome numerical abnormality in spermatozoa[J].同济大学学报（医学版）,2011,32(1):24-27. [点击复制]
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| 摘要: |
| 目的 建立一种应用双色荧光原位杂交技术快速分析正常男性精子XY染色体数目及异常率的方法。方法 采用X、Y号染色体着丝粒探针对7例健康男性精子样本进行荧光原位杂交实验,检测精子XY染色体数目异常情况（非整倍体,双倍体）。结果 计数10 078个精子,杂交率99.00%。其中X染色体杂交率为（49.789±1.346）%,Y染色体杂交率为（48.814±1.296）%,精子中双体类型为XX、XY、YY,其中XX杂交率为（0.110±0.053）%,XY杂交率为（0.222±0.077）%,YY杂交率为（0.094±0.038）%。结论 采用荧光原位杂交技术建立了一种能够快速高效分析精子染色体数目异常的检测方法,可作为精子质量遗传学检测手段,应用于男性不育症的临床检测。 |
| 关键词: 双色荧光原位杂交 精子检测 非整倍体 双倍体 染色体数目畸变 |
| DOI：10.3969/j.issn1008-0392.2011.01.024 |
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| 基金项目:上海市科委基金资助项目(09411960100) |
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| Establishment of a rapid technical assessment of sex chromosome numerical abnormality in spermatozoa |
| LE Wei,QIAN Jun-hai,ZHAO Hua-ying,YUAN Tao,WU Deng-long,ZHANG Jin-fu |
| (Dept.of Urology Surgery,Tongji Hospital,Tongji University,Shanghai 200065,China) |
| Abstract: |
| Objective To establish a rapid method to analyze the numerical abnormality of chromosome X and Y in spermatozoa from the healthy males by using two-color fluorescence in situ hybridization(FISH).Methods Fluorescence in situ hybridization was carried out by using centromeric probes targeting chromosomes,including X and Y upon the sperm samples harvested from 7 healthy men with normal semen quality.The frequency of numerical abnormality(aneuploidy,diploidy) was rated in each sample.Results A total of 10078 sperm cells were counted. The total hybridization rate was 99.00% with (49.789±1.346) % for chromosome X and (48.814±1.296) % for chromosome Y. Abnormality in these samples included XX-, XY-, and YY-typed chromosomes, respectively with incidence of (0.110±0.053) % , (0.222±0.077) % , and (0.094±0.038) %. Conclusion A rapid and efficient method has been established to analyze the numerical abnormality in sperm sex chromosomes by using fluorescence in situ hybridization. This method may be adopted to examining genetically the quality of spermatozoa and to estimate male's infertility. |
| Key words: two-color fluorescence in situ hybridization spermatozoa aneuploidy dipoidy chromosome numerical abnormality |
