| 引用本文： |
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程晓芸,王吉影,李文君,等.2型糖尿病GK大鼠胰岛β细胞凋亡的研究[J].同济大学学报（医学版）,2010,31(5):12-15. [点击复制]
- CHENG Xiao-yun,WANG Ji-ying,LI Wen-jun,et al.Beta cell apoptosis in GK rat of type 2 diabetes[J].同济大学学报（医学版）,2010,31(5):12-15. [点击复制]
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| 摘要: |
| 目的研究2型糖尿病GK大鼠胰岛β细胞凋亡可能机制。方法选择10周龄雄性GK大鼠为2型糖尿病大鼠模型,同源雄性Wistar大鼠为对照组,均以普通饲料喂养12周,定期行OGTT、血脂、空腹胰岛素测定。12周后处死大鼠,光镜下HE染色观察胰岛结构,电镜观察大鼠胰腺组织超微结构的改变,免疫组化法检测胰腺组织Bcl-2及Bax蛋白的表达,TUNEL法检测胰腺组织的β细胞凋亡数。结果与Wistar大鼠比较,GK大鼠血糖呈轻、中度升高（P〈0.05）,尤以餐后血糖升高明显;血清总胆固醇、低密度脂蛋白升高（P〈0.01）,血清甘油三酯降低（P〈0.05）,血清高密度脂蛋白无明显变化（P〉0.05）,血浆胰岛素水平升高（P〈0.01）。透射电镜观察Wistar大鼠的胰岛结构完整,GK大鼠胰岛β细胞呈现凋亡早期改变。GK大鼠Bcl-2蛋白表达低于Wistar大鼠（P〈0.05）,Bax蛋白表达高于Wistar大鼠（P〈0.01）,β细胞凋亡数高于Wistar大鼠（P〈0.01）。结论 GK大鼠早期即可呈现胰岛β细胞凋亡,凋亡相关基因Bcl家族成员间的失衡是导致GK大鼠胰岛β细胞凋亡的可能机制之一。 |
| 关键词: GK大鼠 细胞凋亡 Bcl-2/Bax |
| DOI：10. 3969/j. issn1008 — 0392. 2010.05.004 |
| 投稿时间：2010-07-05 |
| 基金项目:上海市科委科技攻关项目（04DZ19507) |
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| Beta cell apoptosis in GK rat of type 2 diabetes |
| CHENG Xiao-yun,WANG Ji-ying,LI Wen-jun,SHENG Chun-jun,YANG Peng,QU Shen |
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| Abstract: |
| Objective To study the islet beta cell apoptosis in GK rats of type 2 diabetes and its possible mechanisms.Methods Ten weeks old male GK rats were used for type 2 diabetes models and congeneic male Wistar rats as controls.All animals were fed regularly for 12 weeks during which the oral glucose tolerance test（OGTT）,blood lipid and fasting blood sugar（FBS） were determined periodically.At the end of 12 weeks all animal were sacrificed for examination of islet structure and the ultra microstructure of pancreas by transmission electron microscopy.The expression of bcl-1 and bax genes were also detected by using immuno-histochemical technique.Results Compared to Wistar rats,blood sugar rose slightly to moderately in GK rats（P0.05）,but the postprandial blood sugar rose greatly.The level of plasma insulin was also increased in GK rats（P0.01）.Serum levels of total cholesterol and low-density lipoprotein cholesterol（LDL-Ch） were higher in GK rats than those in Wistar rats（P0.01）,but levels of serum triglyceride（TG） in GK rats was lower than that in Wistar rats（P0.05）.There is no significance on high density lipoprotein cholesterol（HDL-Ch） between two groups（P0.05）.By transmission electron microscopy islet structure of Wistar rats was found intact,but islet beta cells apoptosis was observed in GK rats.The levels of Bcl-2 expression was lower in GK rats（P0.05）,but the expression of Bax protein was significantly higher（P0.01）.The number of apoptotic beta cells was more in GK rats than in Wistar rats（P0.01）.Conclusion Apoptosis of islet beta cells ocurrs at early stages of diabetes in GK rats.Imbalance of apoptosis-related genes like members of Bcl family might be involved in the regulation of the apoptosis in beta cells. |
| Key words: Bcl-2/Bax |
