| 摘要: |
| 目的探讨IL-33在大鼠急性胰腺炎（acute pancreatitis,AP）中的作用。方法将64只SD大鼠随机分成急性胰腺炎组（AP组）及假手术组（SO组）,每组分4个时间点（术后3、6、12、24h）,每个时间点8只大鼠。采用4%牛磺胆酸钠胰胆管逆行注射制备AP动物模型,SO组仅行开腹后翻动胰腺。各组处理后于相应时间点留取大鼠的胰腺组织及静脉血,观察AP病理变化并检测血中AMY、TNF-α和IL-33的变化。结果 AP组的AMY和TNF-α以及病理评分均比同时间点SO组显著升高（P〈0.01）,在前6h内随时间逐渐上升（P〈0.05）。在AP发生6h内,AP组IL-33比SO组显著升高（P〈0.01）,呈显著上升趋势（P〈0.05）,且与TNF-α及病理评分呈正相关,与TNF-α相关系数为0.82（P〈0.01）,与病理评分相关系数为0.75（P〈0.05）。6h以后,IL-33呈下降趋势（P〈0.05）,24h时降至与SO组相当。结论 IL-33在AP早期上升,且与TNF-α及病理评分正相关,可能在AP早期起促炎因子的作用。 |
| 关键词: 急性胰腺炎 白细胞介素33 肿瘤坏死因子α 大鼠 |
| DOI：10. 3969/j. issn1008 — 0392. 2010.05.003 |
| 投稿时间：2010-07-07 |
| 基金项目:国家自然科学基金资助项目（30940034) |
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| The significance of changing of serum IL-33 in acute pancreatitis in rats |
| ZHANG Heng-chao,FANG Lin |
| () |
| Abstract: |
| Objective To study the function of IL-33 in rats with acute pancreatitis（AP）.Methods Sixty-four SD rats were randomly divided into AP group and sham operation（SO） group.AP was induced in rats with retrograde infusion of 4% sodium taurocholate into the the pancreastobiliary duct（1ml/kg）.The rats were sacrificed at 3,6,12 and 24 h after operation.The samples of blood serum and pancreas tissue were collected at each time point for detecting AMY,TNF-α and IL-33 levels and distinguishing AP pathological score.Results The levels of AMY,TNF-α and pathological score at all time points in AP group were significantly higher than those in SO group（P0.05）,and the elevated levels were increased with time during the first 6 hour.The level of IL-33 in AP group was significantly higher than that in SO group at 3,6,12h time points（P0.01） although the level of IL-33 in AP group was dramatic increased during first 6h and then droped rapidly afterward.More interestingly,the level of IL-33 was significantly positively correlated with TNF-α level and AP pathological score at the first 6h time point（r=0.82,P0.01 with TNF-α）、（r=0.75,P0.05 with pathological score）.However the level of IL-33 in AP group had no significant difference as compared with that in SO group at 24h time point.Conclusion Elevated IL-33 is significantly positively correlated with TNF-α level and AP pathological score in the early stage of AP indicating IL-33 may play an important role as a pro-inflammatory factor during the early AP development. |
| Key words: acute pancreatitis interleukin-33 tumor necrosis factor alpha rat |
