| 引用本文： |
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陶春华,苏婧玲,黄志刚,等.miR-221异常表达对胃癌细胞增殖和凋亡的影响[J].同济大学学报（医学版）,2010,31(2):44-46. [点击复制]
- TAO Chun-hua,SU Jing-ling,HUANG Zhi-gang,et al.Effects of abnormal expression of miR-221 on proliferation and apoptosis of gastric cancer cell lines[J].同济大学学报（医学版）,2010,31(2):44-46. [点击复制]
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| 摘要: |
| 目的探讨miR-221反义寡核苷酸（miR-221ASO）对胃癌细胞增殖的调控作用。方法转染miR-221ASO，MTT实验观察miR-221ASO对胃癌细胞调控产生的系列效应，采用茎环RT—PCR检测胃癌细胞株SGC-7901和MKN-45中miR-221的表达变化。结果miR-221ASO转染可显著抑制胃癌细胞SGC～7901和MKN-45表达（P=0．027、0．013），miR-221ASO对胃癌细胞的生长抑制率显著减少。结论miR-221ASO对胃癌细胞miR-221水平降低具有靶向特异性．转染miR-221ASO可有效抑制miR-221的促癌效应．利用反义核酸技术的高度特异性开展针对促癌miRNA分子的靶向治疗将可能为胃癌的基因治疗开辟新的途径。 |
| 关键词: 微小RNA 胃肿瘤 靶向治疗 |
| DOI：10.3969/j.issnl008-0392.2010.02.010 |
| 投稿时间：2009-11-19 |
| 基金项目: |
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| Effects of abnormal expression of miR-221 on proliferation and apoptosis of gastric cancer cell lines |
| TAO Chun-hua,SU Jing-ling,HUANG Zhi-gang,LIU Zhan-ju |
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| Abstract: |
| Objective To explore the role played by microRNA-221 on the regulation, of gastric cancer cell proliferation by using antisense MiR-221 ASO. Methods MiR-221 ASO was transfected into gastric cancer cells via liposome,the blocking effect was verified by the down-regulation of MMP7 expression examined by RT-PCR. The effect of miR-221 ASO on the growth of cancer cell lines in vitro was analyzed by MTT assay. Results The expression of miR-221 was down-regulated significantly in gastric cancer cells transfected with miR-221 ASO. Furthermore,the growth of gastric cancer cell lines was inhibited through down-regulating miR-221 ASO. Conclusion The promoting activities of miR-221 on gastric cancer cells can be antagonized effectively by miR-221 ASO. The use of antisense nucleic acid technology for gene therapy of gastric cancer may hold great promise. |
| Key words: miRNA gastric carcinoma target therapy |
