| 引用本文： |
-
颜洁,王新华,翟青新.L-精氨酸促进背根神经节NO合成对糖尿病大鼠机械性痛敏的影响[J].同济大学学报（医学版）,2010,31(1):40-44. [点击复制]
- YAN Jie,WANG Xin-hua,ZHAI Qing-xin.Effects of L-Arginin promotion on nitric oxide expression on mechanical hyperalgesia in diabetic rats[J].同济大学学报（医学版）,2010,31(1):40-44. [点击复制]
|
|
| 摘要: |
| 目的研究L-精氨酸减轻糖尿病大鼠机械性痛敏的作用及可能的机制。方法36只成年雄性SD大鼠随机分为3组（n=12）：正常对照组（CON组）、糖尿病组（DM组）、L-精氨酸（L—Arginin）干预组（L—Arg组）。糖尿病大鼠成模后L—Arg组第l天腹腔注射L—Arginin300mg／kg，每日1次，持续14d。DM组第l天腹腔注射等剂量生理盐水，每日1次，持续14d。CON组在相同时间点腹腔注射等剂量生理盐水，每日1次，持续14d。测定L-arginin注射前（T0）、注射后14d（T1）、21d（T9、28d（T3）测定大鼠机械缩足闽值（paw mechanical withdrawal threshold,PMWT）。L—Arginin注射后28d采用硝酸还原酶法测定背根神经节中一氧化氮（nitrogenmonoxidum，NO）含量、免疫组化法测定背根神经节降钙素基因相关肽（calcitoningene—relatedpeptide，CGRP）表达。结果Tn时间点，DM组PMWT相对于CON组明显降低，在T1、T2、T3时间点较CON组进一步降低（P〈0．05）。L—Arg组在T1、T2、T3时间点相对于DM组PMwT升高（p〈0．05）。DM组背根神经节NO含量较c0N组明显减少，L—Arg组背根神经节N0表达相对DM组明显增加（P〈0．05）。DM组背根神经节cGRP阳性颗粒表达较c0N组减少，给予L—Arginin后背根神经节CGRP表达相对与DM组明显增加（P〈0．05）。结论糖尿病大鼠机械性痛敏增强可能与背根神经节NO和CGRP表达下调有关，L—Arginin可明显促进背根神经节N0合成及CGRP表达，可能是其减轻机械性痛敏机制之一。 |
| 关键词: 糖尿病 痛觉增敏 一氧化氮 降钙素基因相关肽 背根神经节 |
| DOI：10.3969/j.issn1008-0392.2010.01.009 |
|
| 基金项目: |
|
| Effects of L-Arginin promotion on nitric oxide expression on mechanical hyperalgesia in diabetic rats |
| YAN Jie,WANG Xin-hua,ZHAI Qing-xin |
| (Dept.of Pain,East Hospital,Tongji University School of Medicine,Shanghai 200120,China,;Animal Experimental Center,DrumTower Hospital,Nanjing University School of Medicine,Nanjing,Jiangsu Province 210008,China) |
| Abstract: |
| Objective To investigate the role and possible mechanisms of L-Arginin in reducing mechanical hyperalgesia in diabetic rats. Methods Male SD rats were divided into 3 groups randomly (h=12): normal group (CON), diabetic mellitus group (DM), L-Arginin intervention group (L-Arg). L-Arginin (300 mg/kg) was injected intraperitoneally once a day for 2 weeks consecutively in L-Arg group DM rats. The same volume of normal saline was injeceted once a day for 2 weeks in rats of DM group and in CON group at the same time. Paw mechanical withdrawal threshold (PMWT) was measured before L-Arginin injection(T0)and after 14 d(T1), 21 d(T2), 28 d (T3) of L-Arginin injections. After 28 d of L-Arginin injection, nitrogen monoxide (NO) content and calcitonin gene-related peptide (CGRP) in dorsal root ganglion were determined by nitrate reductase method and immunohistochemistry respectively. Results PMWT were significantly decreased at T0, Ti, T2 and T3 in DM group than that in CON group (P<0.05) . PMWT in group L - Arg were increased at T1; T2 and T3 comp ared wi th th at in DM group (P<0.05) . The level s of NO and CGRP in dors al root ganglion were decreased in DM group than that in CON group (P<0.05) . Inj ection of L - Ariginin increased NO c ontent as well as the level of CGRP in dors al root g anglion (P<0.05) . Conclusi on Mechanical hyperalgesia in diabetic rats may be related with the reduction of NO and CGRP expression in doral root ganglion. L-Arginin can significantly promote the synthesis of NO and the CGRP expression in dorsal root ganglion, which may be one of the mechanisms to reduce mechanical hyperalgesia. |
| Key words: diabetic mellitus hyperalgesia NO CGRP dorsal root ganglion |
