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  • 谢荷樱,王陶,赵倩,等.长链非编码RNA CCAT2在不同亚型乳腺癌的表达特征及亚结构分布[J].同济大学学报(医学版),2022,43(5):611-617.    [点击复制]
  • XIE Heying,WANG Tao,ZHAO Qian,et al.Expression and cellular distribution of long non-coding RNA CCAT2 in different subtypes of breast cancer[J].同济大学学报(医学版),2022,43(5):611-617.   [点击复制]
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长链非编码RNA CCAT2在不同亚型乳腺癌的表达特征及亚结构分布
谢荷樱,王陶,赵倩,俞作仁
0
(锦州医科大学上海东方临床医学院,上海200120;同济大学附属东方医院转化医学研究中心,上海200120)
摘要:
目的分析长链非编码RNA结直肠癌相关转录本2(colon cancer-associated transcript 2, CCAT2)在不同亚型乳腺癌的表达和分布,有利于揭示其调控乳腺癌的功能和机制。方法利用TCGA公共数据库,分析乳腺癌患者CCAT2在Luminal型、HER2阳性、三阴性乳腺癌及正常对照组织中的表达水平;通过在线数据库Kaplan-Meier Plotter分析Luminal型和三阴性乳腺癌患者生存率与CCAT2表达水平之间相关性;通过乳腺癌细胞荧光定量PCR分析,检测CCAT2在Luminal型乳腺癌细胞MCF-7、T47D及三阴性乳腺癌细胞Hs578T、SUM159的表达水平;通过细胞质核分离和荧光原位杂交实验,明确CCAT2在不同亚型乳腺癌细胞的亚结构分布。结果CCAT2在Luminal型乳腺癌细胞的表达水平显著低于三阴性乳腺癌细胞(P<0.01)。和正常对照相比,CCAT2在Luminal型和HER2阳性乳腺癌组织异常低表达(P<0.05),且在Luminal型的表达水平与患者生存率呈正相关;相反,CCAT2在三阴性乳腺癌表达水平与患者生存率呈负相关。细胞亚结构定位研究显示,CCAT2在乳腺癌细胞主要分布于细胞核,但其在Luminal型乳腺癌细胞MCF-7、T47D中的细胞质分布比例显著高于在三阴性乳腺癌细胞中的分布。结论内源性CCAT2在Luminal型和三阴性乳腺癌中发挥不同功能,可能与其在两种亚型乳腺癌的表达水平及细胞亚结构分布有关。
关键词:  结直肠癌相关转录本2  乳腺癌  亚型  亚结构分布
DOI:10.12289/j.issn.1008-0392.22217
投稿时间:2022-05-22
基金项目:上海市科学技术委员会基础研究项目(20JC1410400)
Expression and cellular distribution of long non-coding RNA CCAT2 in different subtypes of breast cancer
XIE Heying,WANG Tao,ZHAO Qian,YU Zuoren
(Jinzhou Medical University, Shanghai East Hospital Campus, Shanghai 200120, China;Research Center for Translational Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China)
Abstract:
ObjectiveTo analyze the expression and distribution of long non-coding RNA(lncRNA) colon cancer-associated transcript 2(CCAT2) in different subtype of breast cancer, which will be useful to reveal its function and mechanism in regulating breast cancer. MethodsTCGA public database was applied to determine the expression levels of CCAT2 in Luminal, HER2 positive, triple-negative breast cancer patients and normal control tissues; A online tool of Kaplan Meier Plotter was applied to determine correlations between the overall survival and CCAT2 expression levels in Luminal or triple-negative breast cancer patients; Quantitative RT-PCR analysis was applied to detect the expression of CCAT2 in Luminal breast cancer cell lines MCF-7 and T47D, and triple-negative breast cancer cell lines Hs578T and SUM159; Cytoplasmic & nuclear isolation and in situ hybridization were applied to determine the substructural distribution of CCAT2 in different subtypes of breast cancer cells. ResultsCCAT2 showed lower levels in Luminal breast cancer cells than triple-negative breast cancer cells(P<0.01). Compared with normal control tissues, CCAT2 was downregulated in Luminal and HER2+ subtypes of breast cancer patients(P<0.05). Its expression levels were positively correlated with the overall survival in Luminal breast cancer patients. In contrast, CCAT2 expression showed negative correlation with the overall survival in triple-negative breast cancer patients. Endogenous CCAT2 was mainly distributed in the nucleus of breast cancer cells, but the percentage of cytoplasmic CCAT2 in Luminal subtype of breast cancer cells was significantly higher than that in triple-negative breast cancer cells. ConclusionCCAT2 may play different roles in Luminal and triple-negative breast cancer, which may be related with the difference of its expression level and subcellular distribution in different subtypes of breast cancer.
Key words:  colon cancer-associated transcript 2  breast cancer  subtype  substructure distribution

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