| 引用本文： |
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顾金香,李凯锐,吴家涛,等.Fbxl10通过PI3K途径促进肺癌细胞增殖和侵袭[J].同济大学学报（医学版）,2022,43(3):310-315. [点击复制]
- GU Jinxiang1,LI Kairui,WU Jiatao,et al.Promoting effect of Fbxl10 on proliferation and invasion of lung cancer cells through PI3K pathway[J].同济大学学报（医学版）,2022,43(3):310-315. [点击复制]
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| 摘要: |
| 目的探讨组蛋白去甲基化酶Fbxl10对肺癌细胞增殖、迁移和侵袭的影响及潜在的作用机制。方法体外培养肺腺癌细胞株H1299，设空白对照组(Ctrl组)，阴性质粒对照组(NC组)，过表达组(Fbxl10OE组)和干扰组(siFbxl10组)，均采用脂质体法转染至肺腺癌H1299细胞株；分别利用细胞克隆形成实验及MTT实验检测Fbxl10基因对肺腺癌细胞株增殖的影响；细胞划痕实验和Transwell迁移和侵袭实验检测Fbxl10基因对肺腺癌细胞株迁移和侵袭能力的作用；qRT-PCR和蛋白免疫印记实验技术检测转染后肺腺癌细胞株Fbxl10和PI3K的mRNA水平以及相关蛋白的表达情况。结果与NC组比较，在肺腺癌H1299细胞株中过表达Fbxl10，能促进肺癌细胞的增殖、迁移和侵袭能力(P<0.05)；qRT-PCR结果显示，过表达Fbxl10时，Fbxl10 mRNA与PI3K mRNA的过表达组均高于NC组(P<0.05)，蛋白免疫印记实验结果显示，过表达Fbxl10时，Fbxl10蛋白与PI3K蛋白的表达也明显高于NC组，差异有统计学意义(P<0.05)。结论Fbxl10过表达能促进肺癌细胞的增殖、迁移和侵袭能力，机制可能与激活了PI3K信号通路有关。 |
| 关键词: Fbxl10 PI3K 肺腺癌 增殖 侵袭 |
| DOI：10.12289/j.issn.1008-0392.22044 |
| 投稿时间：2022-02-03 |
| 基金项目:安徽省高等学校自然科学研究项目（KJ2021A0725）；安徽省科技厅面上项目（1708085MH231） |
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| Promoting effect of Fbxl10 on proliferation and invasion of lung cancer cells through PI3K pathway |
| GU Jinxiang1,LI Kairui,WU Jiatao,WANG Luyao,WU Shiwu |
| (Department of Pathology, Bengbu Medical College, Bengbu 233034, Anhui Province, China;Department of Pathology, The First People’s Hospital Affiliated to Bengbu Medical College, Bengbu 233034, Anhui Province, China) |
| Abstract: |
| ObjectiveTo investigate the effects of histone demethylase Fbxl10 on proliferation, migration and invasion of lung adenocarcinoma cells and its potential mechanism. MethodsLung adenocarcinoma H1299 cells were cultured in vitro and divided into control group (no treatment), NC group (transfected with negative plasmid), Fbxl10OE-overexpressing group (transfected with Fbxl10OE) and interfering group (transfected with siFbxl10). Cell proliferation was detected by clonal formation assay and MTT assay, cell migration and invasion ability were examined by cell wound healing assay and Transwell test. The expressions of Fbxl10 and PI3K mRNA and protein was detected with qRT-PCR and Western blotting, respectively. ResultsCompared to NC group, the proliferation, migration and invasion of H1299 cells in overexpressing Fbxl10 group were enhanced (all P<0.05). The results of qRT-PCR showed that the expression of Fbxl10 mRNA and PI3K mRNA in Fbxl10-overexpressing group was higher than that in NC group (P<0.05). Western blotting showed that the expression of Fbxl10 protein and PI3K protein in Fbxl10-overexpressing group was also significantly higher than that in NC group (P<0.05). ConclusionOverexpression of Fbxl10 promotes the proliferation, migration and invasion of lung cancer cells, which is associated with the activation of PI3K signaling pathway. |
| Key words: Fbxl10 PI3K lung adenocarcinoma proliferation invasion |
