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  • 刘古月,闫佩毅,涂焕平,等.乳腺癌患者ANXA6基因的表达及其病理意义[J].同济大学学报(医学版),2021,42(5):678-684.    [点击复制]
  • LIU Gu-yue,YAN Pei-yi,TU Huan-ping,et al.Expression of ANXA6 gene and its clinicopathological significance in breast cancer patients[J].同济大学学报(医学版),2021,42(5):678-684.   [点击复制]
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乳腺癌患者ANXA6基因的表达及其病理意义
刘古月,闫佩毅,涂焕平,冯一鸣,蔡骁垚,金姝
0
(南华大学衡阳医学院,湖南 衡阳421001; 同济大学附属普陀人民医院外科,上海200060;同济大学附属普陀人民医院检验科,上海200060;同济大学附属普陀人民医院病理科,上海200060)
摘要:
目的探讨ANXA6基因在人乳腺癌组织中的表达及其与患者临床病理特征的相关性。方法选择2006年11月—2010年12月收治并病理确诊的55例乳腺癌患者组织标本进行石蜡包埋,采用免疫组织化学方法检测55例乳腺癌患者的癌组织及癌旁组织中ANXA6基因的表达,以RT-PCR技术和Western印迹法验证ANXA6在乳腺癌组织及癌旁组织中的表达,分析ANXA6基因表达与患者临床特征的关系。结果55例患者癌与癌旁组织中ANXA6基因的阳性表达分别为96.3%(53/55)和52.7%(29/55),差异具有统计学意义(P<0.05);癌组织中ANXA6与自噬相关蛋白LC3B、P62、TFEB表达均高于癌旁组织(P<0.05)。ANXA6基因在癌组织中的表达阳性率与肿瘤大小、临床病理分期、PR表达有关(P<0.05);而与有无淋巴结转移、5年生存率、年龄、组织学分级、ER表达、HER-2表达均无关(P>0.05)。ANXA6基因在癌组织中表达阳性率与LC3B表达、TFEB表达相关(P<0.05),与P62表达无关(P>0.05)。癌组织中ANXA6基因表达上调率与肿瘤大小、有无淋巴结转移、临床病理分期、5年生存率、年龄、组织学分级、PR表达、HER-2表达,TFEB表达有关(P<0.05),与ER表达、LC3B及P62表达无关(P>0.05)。结论ANXA6基因在乳腺癌组织中高表达,其在癌组织中表达阳性率与肿瘤大小、临床病理分期、PR表达、LC3B表达、TFEB表达有关,其在癌组织中表达上调率与肿瘤大小、有无淋巴结转移、临床病理分期、5年生存率、年龄、组织学分级、PR表达、HER-2表达,TFEB表达有关,对于乳腺癌可能具有一定的辅助诊断价值。
关键词:  ANXA6基因  乳腺癌; 免疫组化; 细胞自噬
DOI:10.12289/j.issn.1008-0392.21194
投稿时间:2021-05-12
基金项目:上海市普陀区科委-卫生系统自主创新科研项目(ptkwws201825);上海市普陀区卫生健康系统科技创新项目(ptkwws202105)
Expression of ANXA6 gene and its clinicopathological significance in breast cancer patients
LIU Gu-yue,YAN Pei-yi,TU Huan-ping,FENG Yi-ming,CAI Xiao-Yao,JIN Shu
(Hengyang Medical College of Nanhua University, Hengyang 421001, Hunan Province, China; Dept. of Surgery, Putuo Peoples Hospital, School of Medicine, Tongji University, Shanghai 200060, China;Dept. of Laboratory, Putuo Peoples Hospital, School of Medicine, Tongji University, Shanghai 200060, China;Dept. of Pathology, Putuo Peoples Hospital, School of Medicine, Tongji University, Shanghai 200060, China;Hengyang Medical College of Nanhua University, Hengyang 421001, Hunan Province, China; Dept. of Laboratory, Putuo Peoples Hospital, School of Medicine, Tongji University, Shanghai 200060, China)
Abstract:
ObjectiveTo investigate the expression of ANXA6 gene in human breast cancer and its correlation with clinicopathological features. MethodsParaffin embedded tissue samples were taken from 55 patients with breast cancer from November 2006 to December 2010. Immunohistochemistry was used to detect the expression of ANXA6 gene in breast cancer tissues and para-cancerous tissues. The expression of ANXA6 in breast cancer tissues and para-cancerous tissues was verified by RT-PCR and Western blotting. The relationship between ANXA6 gene expression and clinicopathological features of patients was analyzed. ResultsThe positive expression of ANXA6 gene was 96.3%(53/55) in cancer tissues and 52.7%(29/55) in para-cancerous tissues, respectively(P<0.05). The expressions of ANXA6 and autophagy-related proteins LC3B, P62 and TFEB in cancer tissues were higher than those in para-cancerous tissues(P<0.05). The expression of ANXA6 gene was correlated with tumor size, clinicopathological stage, and PR expression(P<0.05); while not correlated with lymph node metastasis, 5-year survival rate, age, histological grade, ER and HER-2 expression(P>0.05). The expression of ANXA6 was correlated with LC3B and TFEB(P<0.05), but not with P62(P>0.05). The up-regulation rate of ANXA6 gene expression in cancer tissues was correlated with tumor size, lymph node metastasis, clinicopathological stage, 5-year survival rate, age, histological grade, PR expression, HER-2 expression and TFEB expression(P<0.05), but not with ER expression, LC3B and P62 expression(P>0.05). ConclusionANXA6 gene is highly expressed in breast cancer tissues, which is correlated with tumor size, clinicopathological stage, PR expression, LC3B expression and TFEB expression. The up-regulation rate in tumor tissues is corelated with tumor size, lymph node metastasis, clinicopathological stage, 5-year survival rate, age, histological grade, PR expression, HER-2 expression and TFEB expression. It may have some diagnostic value for breast cancer.
Key words:  ANXA6 gene  breast cancer  immunohistochemistry  autophagy

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