| 引用本文： |
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李伟,王佳怡,肖春媛,等.IL-4通过促进CD30分子表达抑制类风湿关节炎患者T细胞功能的研究[J].同济大学学报（医学版）,2021,42(4):528-533. [点击复制]
- LI Wei,WANG Jia-yi,XIAO Chun-yuan,et al.IL-4 inhibits T cell function in patients with rheumatoid arthritis through promoting CD30 expression[J].同济大学学报（医学版）,2021,42(4):528-533. [点击复制]
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| 摘要: |
| 目的观察IL-4和CD30在类风湿关节炎（rheumatoid arthritis, RA）中的潜在关联，并探讨其对T细胞促炎细胞因子分泌的影响。方法选取46例活动期RA患者（RA组）及41例健康人（对照组）为研究对象，其中RA患者DAS28评分3.89～7.41分，平均（4.23±1.42）分。ELISA法测定RA血清中IL-4和sCD30含量并分析二者相关性；流式细胞术分析重组人TIL-4蛋白对PBMC来源的T细胞表面CD30表达的影响，观察IL-4刺激后CD30+T及CD30-细胞内IFN-γ、IL-17的水平变化。结果与健康对照组相比，RA患者血清中sCD30水平显著升高（P<0.0001），IL-4水平也有升高（P<0.01)，Pearson相关性分析显示二者存在正相关。在抗CD3、抗CD28活化培养条件下，重组IL-4蛋白可促进RA患者外周血中T细胞表达CD30分子增加，增加程度与IL-4浓度呈正比。细胞内荧光染色显示，RA患者CD30+T细胞内IL-17较对照组明显升高（P<0.05）。重组IL-4蛋白能降低RA及对照组外周血中CD30+T细胞分泌IFN-γ、IL-17，而对CD30-T细胞内细胞因子的分泌无明显影响。结论IL-4可能通过促进T细胞上CD30分子表达降低促炎细胞因子产生，在RA中发挥免疫调节作用。 |
| 关键词: 白细胞介素-4 CD30分子 类风湿关节炎 T细胞 |
| DOI：10.12289/j.issn.1008-0392.20481 |
| 投稿时间：2020-11-09 |
| 基金项目:上海市浦东新区科委创新基金（PKJ2016-Y61）；同济大学中央高校基本科研项目（22120180467） |
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| IL-4 inhibits T cell function in patients with rheumatoid arthritis through promoting CD30 expression |
| LI Wei,WANG Jia-yi,XIAO Chun-yuan,LU Tian-lin,HUANG Xin-fang,HAN Jie |
| (Dept. of Rheumatology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China) |
| Abstract: |
| Objective
To investigate the potential association of IL-4 and CD30 in rheumatoid arthritis and to explore their effects on T cell pro-inflammatory cytokine secretion. MethodsForty-six active RA patients with an average DAS28 score of 4.23±1.42(3.89-7.41) (RA group) and forty-one healthy subjects(control group) were enrolled in the study. The serum levels of IL-4 and sCD30 were measured by ELISA, the expression of CD30 on T cells derived from PBMC was analyzed by flow cytometry, the levels of IFN-γ and IL-17 in CD30+ T cells and CD30- T cells were determined after IL-4 stimulation. ResultsCompared with the control group, the serum sCD30 and IL-4 levels in RA patients were significantly increased (P<0.0001 and P<0.01). Pearson correlation analysis showed that there was a positive correlation between them. Under the stimulation of anti-CD3 and anti-CD28, recombinant human IL-4 protein significantly increased the expression of CD30 on T cells of RA patients in a dose-dependent manner(P＜0.05). Intracellular fluorescence staining showed that IL-17 in CD30+ T cells of RA patients was significantly higher than that in healthy controls(P<0.05). IL-4 decreased the secretion of IFN-γ and IL-17 by CD30+ T cells in RA and control group but had no effect on CD30-T cells. ConclusionIL-4 may play an immunomodulatory role in RA by promoting the expression of CD30 on T cells and decreasing the production of pro-inflammatory cytokines. |
| Key words: interleukin-4 CD30 molecule rheumatoid arthritis T cell |
