| 引用本文： |
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夏忠源,秦家骏,陈先震.间断束缚和单次束缚对抑郁小鼠模型建立的影响[J].同济大学学报（医学版）,2021,42(3):299-305. [点击复制]
- XIA Zhong-yuan,QIN Jia-jun,CHEN Xian-zhen.Comparison of intermittent restrain and constant restrain in establishing mice model of depression[J].同济大学学报（医学版）,2021,42(3):299-305. [点击复制]
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| 摘要: |
| 目的比较两种不同束缚频次对小鼠抑郁模型建立效果的影响。方法将75只雄性C57BL16J小鼠分为对照（Con）组，单次束缚组（constant group, CR）组和间断束缚（intermittent restraint, IR）组。在每日总束缚时长相同的情况下分别给予不同频次的束缚刺激建立抑郁小鼠模型，通过观察行为学以及组织学等方面表现对两种方法建立的抑郁小鼠模型进行评价。行为学检测包括SPT、TST、FST和OFT。组织学检测包括AAV病毒示踪神经元形态、Western印迹法检测小鼠前额叶PTEN和海马BDNF表达，ELISA法检测炎症因子水平。结果经过6周的束缚，两束缚组小鼠均表现出不同程度的体质量下降、SPF值下降，FST与TST平均不动时间增加、OFT中央区域时间减少现象。且在束缚周期内，两观察组小鼠体质量和4项行为学指标均出现组间差异。IR组PTEN表达上调较CR组明显，且BDNF表达较CR组减少；IR组小鼠PFC区锥体神经元突触数量少于CR组（P=0.01），神经元胞体变小；IR组小鼠海马小胶质细胞数量明显增多。结论在束缚总时长相同的情况下，间断束缚与单次束缚均可以成功建立抑郁小鼠模型。间断束缚更容易引起小鼠抑郁样表现。 |
| 关键词: 抑郁症 束缚应激 动物模型 行为学检测 小鼠 |
| DOI：10.12289/j.issn.1008-0392.21020 |
| 投稿时间：2021-01-24 |
| 基金项目:上海市卫生健康委员会科研项目（201940126） |
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| Comparison of intermittent restrain and constant restrain in establishing mice model of depression |
| XIA Zhong-yuan,QIN Jia-jun,CHEN Xian-zhen |
| (Dept. of Neurosurgery, Shanghai Tenth Peoples Hospital, Clinical School of Nanjing Medical University, Shanghai 200072, China) |
| Abstract: |
| ObjectiveTo compare the effect of constant restrain stress and intermittent restrain stress in establishing mice model of depression. MethodsSeventy five C57BL/6J mice were randomly divided into control（Con） group, constant restrain（CR） group and intermittent restraint（IR） group with 25 mice in each group. Mice were exposed to different frequency of restrain stress to establish a depression model with the same total daily restraint time. The behavioral tests（SPT, TST, FST and OFT) and histological findings were compared among the 3 groups. Neuron morphology were traced by AAV virus; the expression of PTEN in prefrontal cortex and BDNF in hippocampus were detected by Western blotting, and the levels of inflammatory factors were detected by ELISA. ResultsAfter 6 weeks of restrain, the body weight and sucrose preference of restrained mice decreased, the mean immobility time of FST and TST increased, and central time in OFT decreased. There were significant differences in body weight and in four behavioral tests between the IR and CR groups. The expression level of PTEN in IR group was significantly higher than that in CR group. BDNF expression in IR group was lower than that in CR group. The number of synapses in PFC area of IR group was less than that in CR group（P=0.01). The number of microglia in hippocampus of IR group was significantly increased. ConclusionMice model of depression has been successfully established through both intermittent restraint and consistent restrain. Intermittent restraint is more likely to induce depression-like behavior in mice compared with constant restrain. |
| Key words: depression restrain stress animal models behavioral tests mice |
