LU Yu-xia,LI Xin,AI Zi-sheng,et al.Analysis of intestinal microflora in patients with chronic hepatitis B by 16s rDNA amplicon sequencing[J].同济大学学报（医学版）,2021,42(2):206-213. [点击复制]
Analysis of intestinal microflora in patients with chronic hepatitis B by 16s rDNA amplicon sequencing
LU Yu-xia,LI Xin,AI Zi-sheng,LIANG Ping,YANG Chang-qing
(Dept. of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, China; Dept. of Infectious Diseases, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, China;Dept. of Medical Statistics, School of Medicine, Tongji University, Shanghai 200092, China;Clinical Laboratory, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, China)
Abstract:
ObjectiveTo analyze the intestinal microflora in patients with chronic hepatitis B（CHB) with 16s rDNA amplification sequencing. MethodsSixty CHB patients admitted in the infection and digestive departments of Shanghai Tongji Hospital during 2017—2018, and 30 healthy subjects were enrolled in the study. Feces samples were collected from CHB patients and healthy controls; 16s rDNA amplicon sequencing technique was applied to analyze the intestinal microflora and the results were compared between CHB patients and healthy subjects. ResultsCompared with CHB group, intestinal flora in healthy controls was richer, more diverse and more uniform. At phylum level, the flora were mainly Firmicutes and Bacteroidetes in both groups; while the healthy control group had a unique Planctomycetes. At class level, there were unique Melainabacteria and OM190 in healthy controls; CHB group has much more Bacilli（P=0.000 999). At order level, there were unique Actinomycetales and Pseudomonadales in CHB group; and unique Gastranaerophilales in healthy control group; The Lactobacillales in CHB group was more than that in healthy controls（P=0.000 999); Caulobacterales, Desulfovibrionales, Rhizobiales in CHB group were less than those in healthy controls（P=0.000 999, 0.045 954, 0.000 003 57). At family level, there were unique Actinomycetaceae, Comamonadaceae and Moraxellaceae in CHB group; Defluviitaleaceae, Streptococcaceae and Lachnospiraceae in CHB group were more than those in healthy controls（P=0.000 067 3, 0.000 999, 0.018 981), while Phyllobacteriaceae, Caulobacteraceae and Acidaminococcaceae in CHB group were less than those in healthy controls（P=0.000 003 57, 0.000 999, 0.039 96). At genus level, there were twelve unique bacterial genera in CHB group and five in healthy controls; Lachnospiraceae UCG-010 and Mesorhizobium in CHB group were less than those in healthy controls（P=0.033 966, 0.000 003 57). Following bacteria genera in CHB group were more than those in healthy controls： Ruminococcus l, Eggerthella, Lachnospiraceae NC2004 group, Lachnospiraceae FCS020 group, Clostridium innocuum group, Defluviitaleaceae UCG-011, Oscillibacter, Blautia（P=0.009 99, 0.004 995, 0.030 969, 0.011 988, 0.027 972, 0.000 067 35, 0.000 969, 0.019 98). There were significant differences in intestinal flora structure among CHB patients and less differences among the healthy subjectss. ConclusionCompared with CHB group, intestinal flora in healthy controls is richer, more diverse and more uniform. In phylum, class, order, family and genus levels, intestinal flora in CHB group and healthy controls have their unique and different bacteria strains.
