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  • 柯福彬,Anil Kumar Yadav,谢晓恬,等.再生障碍性贫血患儿骨髓T-bet、GATA3蛋白和外周血细胞因子表达及意义[J].同济大学学报(医学版),2021,42(1):80-84.    [点击复制]
  • KE Fu-bin,Anil KumarYadav,XIE Xiao-tian,et al.Expression on T-bet, GATA3 protein in bone marrow and serum cytokines levels in children with acquired aplastic anemia[J].同济大学学报(医学版),2021,42(1):80-84.   [点击复制]
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再生障碍性贫血患儿骨髓T-bet、GATA3蛋白和外周血细胞因子表达及意义
柯福彬,AnilKumarYadav,谢晓恬,乔晓红
0
(同济大学附属同济医院儿科,上海200065;同济大学附属同济医院儿科,上海200066;同济大学附属同济医院儿科,上海200067;同济大学附属同济医院儿科,上海200068)
摘要:
目的检测调节Th1/Th2细胞分化的T-bet,GATA3蛋白在不同程度获得性再生障碍性贫血患儿骨髓中的表达,同时比较其治疗前外周血IL-2、IL-4、IFN-γ细胞因子水平,探讨获得性再生障碍性贫血的免疫病理机制。方法将49例获得性再生障碍性贫血患儿根据临床表现分为非重型组和重型与极重型组。利用免疫组织化学方法检测骨髓病理切片中T-bet,GATA3蛋白表达。酶联免疫吸附法比较细胞因子IL-2、IL-4、IFN-γ水平。结果T-bet在重型与极重型组AOD值0.0170(0.0140~0.0210)显著高于非重型组0.0060(0.0051~0.0064),P=0.000。GATA3在重型与极重型组和非重型组表达差异无统计学意义(0.0053±0.0010,0.0052±0.0009,P=0.846)。IL-2及IFN-γ在重型与极重型组显著高于非重型组(P=0.000,P=0.007),IL-4两组比较差异无统计学意义(P=0.289)。结论骨髓衰竭重的获得性再生障碍性贫血患儿其骨髓T-bet蛋白表达明显增高,外周血TH1细胞相关因子水平增高,调节Th1的T-bet蛋白在再生障碍性贫血的免疫病理机制中可能起着一定作用。
关键词:  再生障碍性贫血  免疫组织化学  Th1/Th2细胞  T-bet  GATA3
DOI:10.12289/j.issn.1008-0392.20129
投稿时间:2020-03-19
基金项目:国家自然科学基金面上项目(81670119)
Expression on T-bet, GATA3 protein in bone marrow and serum cytokines levels in children with acquired aplastic anemia
KE Fu-bin,Anil KumarYadav,XIE Xiao-tian,QIAO Xiao-hong
(Dept. of Pediatrics, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China)
Abstract:
ObjectiveTo investigate the expression of transcription factors T-bet and GATA3 in bone marrow, and IL-2, IL-4 and IFN-γ levels in peripheral blood of children with acquired aplastic anemia. MethodsForty nine children with acquired aplastic anemia were enrolled in the study, including 25 non-severe cases (NSAA group and 24 severe and very severe cases (SAA/VSAA group). Immunohistochemical staining was used to detect T-bet and GATA3 protein expressions in bone marrow pathological sections. Enzyme linked immunosorbent assay (ELISA) was used to determine serum levels of IL2, IL4 and IFN-γ. ResultsThe AOD value of T-bet in SAA/VSAA group was 0.0170 (0.0140-0.0210), which was significantly higher than that in NSAA group [0.0060(0.0051-0.0064, P=0.000]. There was no significant difference in the expression of GATA3 between the two groups (P=0.846). Serum IL-2 and IFN-γ levels were significantly higher in the SAA/VSAA group than those in NSAA group (P=0.000, P=0.007). There was no significant difference in IL-4 levels between the two groups (P=0.289). ConclusionThe expression of T-bet protein in the bone marrow of aplastic anemia patients with severe bone marrow failure is significantly increased, and the levels of Th1 cytokines in peripheral blood are also increased, indicating that T-bet protein regulating Th1 may play a role in the immunopathological mechanism of aplastic anemia.
Key words:  aplastic anemia  immunohistochemistry  Th1/Th2 cells  T-bet  GATA3

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