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  • 陈荣梅,蒋伟东,蒋备战.硒对氟斑牙发生的拮抗作用及其对Beclin1表达的影响[J].同济大学学报(医学版),2020,41(4):426-430.    [点击复制]
  • CHEN Rong-mei,JIANG Wei-dong,JIANG Bei-zhan.The antagonistic effect of selenium on dental fluorosis and the expression of beclin1 in mice[J].同济大学学报(医学版),2020,41(4):426-430.   [点击复制]
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硒对氟斑牙发生的拮抗作用及其对Beclin1表达的影响
陈荣梅,蒋伟东,蒋备战
0
(同济大学口腔医学院·同济大学附属口腔医院儿童口腔医学教研室,上海牙组织修复与再生工程技术研究中心,上海 200072;广西医科大学附属口腔医院口腔颌面外科教研室,南宁 530021)
摘要:
目的 研究硒对小鼠氟斑牙发生的拮抗作用,并检测其对自噬相关基因Beclin1表达的影响。方法 将36只2月龄费城癌症研究所(institute of cancer research, ICR)雄鼠,随机分为3组,每组12只,建立饮水型氟斑牙模型,用亚硒酸钠(Na2SeO3)进行干预,具体分组为: 对照组(ddH2O)、氟化钠组(200mg/L NaF)、硒组(2mg/L Na2SeO3+200mg/L NaF),喂养8周后,观察氟斑牙发生情况,记录其长度并计算小鼠下颌切牙增长率;分离下颌骨后,进行micro-CT扫描,测量小鼠切牙唇侧硬组织厚度;H-E染色观察成釉细胞的变化,免疫组化方法检测Beclin1的表达。结果 氟化钠组氟斑牙检出率为100%,表现为小鼠切牙白垩色改变;硒组未发现明显氟斑牙症状,形态与色泽介于正常与极轻度之间;micro-CT结果显示,氟化钠组切牙唇侧硬组织厚度比对照组薄,硒组介于两者之间(P<0.01);H-E 结果显示,氟化钠组成釉细胞排列紊乱,极性消失;而对照组与硒拮抗组成釉细胞呈栅栏状,排列整齐规则;免疫组化结果显示,3组中成釉细胞从分泌期到成熟期Beclin1的阳性表达均呈逐渐增强的表达趋势,且Beclin1在氟化钠组表达明显强于正常组,硒组介于两者之间。结论 2mg/L硒离子浓度对氟斑牙的发生有一定拮抗作用;其作用机制可能是通过抑制成釉细胞自噬的发生,进而减轻过量氟对小鼠切牙毒性影响。
关键词:  氟斑牙  亚硒酸钠  细胞自噬  Beclin1  成釉细胞
DOI:10.16118/j.1008-0392.2020.04.004
投稿时间:2020-03-21
基金项目:上海市科学技术委员会医学引导项目(18411969500)
The antagonistic effect of selenium on dental fluorosis and the expression of beclin1 in mice
CHEN Rong-mei,JIANG Wei-dong,JIANG Bei-zhan
(Dept. of Pediatric Dentistry, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China;Dept. of Oral and Maxillofacial Surgery, Stomatology Hospital of Guangxi Medical University, Nanning 530021, Chin)
Abstract:
Objective To investigate the effect of selenium on the development of dental fluorosis in mice and mechanism. autophagy-related gene of Beclin1. Methods Thirty-six ICR male mice aged 2-months were randomly divided into three groups with 12 in each group: ddH2O was given to mice in control group, 200mg/L sodium fluoride(NaF) was given to model group and 2mg/L sodium selenite(Na2SeO3) and 200mg/L NaF was given to treatment group. After 8 weeks of treatment, the occurrence of dental fluorosis and the length of incisors was observed. Then the mandibles were separated, the thickness of hard tissues on the labial side of the incisors was detected by micro-CT 3D-reconstruction after PFA routinely fixed. The morphological changes in ameloblasts were examined with H-E staining and the expression of autophagy-related gene Beclin1 was detected with immunohistochemical staining. Results The rate of dental fluorosis was 100% in the model group, showing chalk-colored changes in mice mandibular incisor; while no fluorosis was found in the selenium treatment group, the shape and color of the incisors were between normal and mild changes. Under micro-CT 3D-reconstruction, the thickness of the hard tissue on the lingual side of the incisor in model group was thinner compared to the control group, while that of the selenium treatment group was between above two groups(P<0.01). As shown in the H-E staining, the arrangement of ameloblasts was disordered and the polarity disappeared in the model group; while the ameloblasts presented a fence-like arrangement in the other two groups. Immunohistochemical results revealed that the expression of Beclin1 showed a gradually increasing trend from pre-secretion to maturation stage of ameloblasts in all three groups, but the expression was strongest in model group, followed by treatment group, that of control group was weakest. Conclusion Selenium has a certain antagonistic effect on dental fluorosis in mice, which may be associated with inhibiting ameloblasts autophagy.
Key words:  dental fluorosis  selenium  cell autophagy  Beclin1  ameloblast

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