| 引用本文： |
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李森,杨焱,黄小流,等.桑黄提取物改善PM2.5暴露诱导的SD大鼠氧化应激及肾损伤[J].同济大学学报（医学版）,2020,41(3):291-295. [点击复制]
- LI Sen,YANG Yan,HUANG Xiao-liu,et al.Protective effect of Inonotus Sanghuang extract on PM2.5 exposure-induced oxidative stress and kidney damage in rats[J].同济大学学报（医学版）,2020,41(3):291-295. [点击复制]
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| 摘要: |
| 目的探究桑黄提取物（Inonotus Sanghuang extract， ISE）对细颗粒物（PM2.5）暴露所致SD大鼠氧化应激和肾功能损伤的作用。方法应用人工气候环境暴露设备，将4周龄雄性SD大鼠随机分为3组，FA组置于对照仓，PM组和ISE组置于PM2.5暴露仓，其中ISE组在每天暴露前给予ISE水悬液灌胃。暴露共持续12周，每周检测一次24 h尿流率，最后收集大鼠尿液、血清和肾组织标本。检测尿液和血清中肌酐、尿素氮含量，计算肾小球滤过率；并用ELISA法检测血清胱抑素C（cystatin C， Cys-C）和尿液β2微球蛋白（β-2-microglobulin， β2-MG）水平，以及血清和肾组织的丙二醛（malondialdehyde， MDA）、超氧化物歧化酶（superoxide dismutase， SOD）和总谷胱甘肽（total glutathione， T-GSH）水平；肾组织切片H-E染色观察肾脏的病理情况。结果暴露第3周起至暴露结束，PM组大鼠的尿流率持续高于FA组，ISE组尿流率较PM组降低而接近于FA组。暴露12周后，与FA组相比，PM组肾损伤指标血清Cys-C和尿液β2-MG升高，氧化应激标志物MDA水平升高，抗氧化应激标志物SOD和T-GSH明显降低；ISE组血清Cys-C和尿液β2-MG低于PM组，同时MDA水平降低，T-GSH水平升高。肾组织切片H-E染色显示，PM组大鼠出现肾小管和肾小球损伤，ISE组与FA组情况相近，未见明显变化。结论亚慢性暴露于PM2.5导致SD大鼠肾功能损伤，而ISE通过发挥其抗氧化能力对PM2.5暴露所致的肾损伤产生保护作用。 |
| 关键词: PM2.5 肾损伤 桑黄 氧化损伤 |
| DOI：10.16118/j.1008-0392.2020.03.004 |
| 投稿时间：2020-01-20 |
| 基金项目:上海市气象与健康重点实验室开放基金（QXJK201612） |
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| Protective effect of Inonotus Sanghuang extract on PM2.5 exposure-induced oxidative stress and kidney damage in rats |
| LI Sen,YANG Yan,HUANG Xiao-liu,LUO Xiao-li,WU Di,LI Jue |
| (Tongji University School of Medicine, Shanghai 200092, China;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Shanghai 201106, China) |
| Abstract: |
| ObjectiveTo investigate the effect of Inonotus Sanghuang extract（ISE) on oxidative stress and kidney function damage in SD rats induced by PM2.5 exposure. MethodsThirty male SD rats aged 4 weeks were randomly divided into three groups. A versatile aerosol concentration enrichment system（VACES) was used for long-term whole-body exposures, FA group was exposed to filtered air, PM group and ISE group were exposed to concentrated ambient PM2.5 for 12 weeks. The ISE water suspension was given to ISE group by gavage before each exposure. The 24-hour urinary flow rate was measured weekly. After the last exposure, the urine, serum and renal tissue samples were taken. The concentration of creatinine and urea nitrogen were measured and the glomerular filtration rate was calculated. The serum Cys-C and urinary β2-MG levels were measured; the MDA, SOD and T-GSH levels in serum and renal tissue were determined by ELISA; and the renal pathology was observed by HE staining. ResultsThe urinary flow rate of PM group was higher than that of FA group; compared to PM group the urinary flow rate in ISE group was decreased and close to that in FA group at the later stage of exposure. After exposure, levels of serum Cys-C, urine β 2-MG and MDA in the PM group were increased, while SOD and T-GSH levels were significantly lower than those in the FA group. Meanwhile, serum Cys-C, urine β 2-MG and MDA levels in ISE group were significantly lower than PM group, while T-GSH levels were significantly higher. H-E staining showed that damage of renal tubules and glomerulus occurred in the PM group，and no significant difference was observed in the ISE group compared with FA group. ConclusionISE has a protective effect on kidney injure in SD rats after PM2.5 exposure, which may be related to the its antioxidant capacity. |
| Key words: PM2.5 kidney damage Inonotus Sanghuang oxidative damage |
