| 引用本文： |
-
陈胜广,马俊花,王菁楠,,等.环丙沙星-固体脂质纳米粒的制备及抗菌效果[J].同济大学学报（医学版）,2019,40(6):815-820. [点击复制]
- CHEN Sheng-guang,MA Jun-hua,WANG Jing-nan,et al.Ciprofloxacin-loaded solid lipid nanoparticles： preparation, characterization and antibacterial activity[J].同济大学学报（医学版）,2019,40(6):815-820. [点击复制]
|
|
| 摘要: |
| 目的 制备环丙沙星固体脂质纳米粒并检测其抑菌效果。方法 以胆固醇为脂质，以吐温80为表面活性剂，采用乳化-低温固化法制备固体脂质纳米粒并对其进行表征，包括粒径、Zeta电位、载药量、包封率、分散性以及体外缓释。使用二倍稀释法测定药物对大肠杆菌的最低抑菌浓度。结果 透射电镜扫描可见环丙沙星-固体脂质纳米粒粒径呈球形，直径40~70nm；Zeta电位（-21.8±1.3） mV；包封率为77.54%；载药量31.10%；紫外-可见光谱见纳米粒中环丙沙星280nm处特征性吸收波峰；体外缓释72h的累计释放度为78.6%。环丙沙星固体脂质纳米粒的最低抑菌浓度为0.8μg/mL，环丙沙星最低抑菌浓度为1.6μg/mL。结论 采用乳化-低温固化法成功制备环丙沙星固体脂质纳米粒，方法简便。固体脂质纳米可提高环丙沙星抑菌效果。 |
| 关键词: 环丙沙星 固体脂质纳米粒 表征 最低抑菌浓度 |
| DOI：10.16118/j.1008-0392.2019.06.009 |
| 投稿时间：2019-03-23 |
| 基金项目:上海市浦东新区公利医院青年项目（2017YQNJJ-01） |
|
| Ciprofloxacin-loaded solid lipid nanoparticles： preparation, characterization and antibacterial activity |
| CHEN Sheng-guang,MA Jun-hua,WANG Jing-nan,ZHU Hong-ling,GU Ming-jun |
| (Dept. of Endocrinology & Metabolism, Gongli Hospital, Shanghai Pudong New Area, Shanghai 200135, China) |
| Abstract: |
| Objective To prepare ciprofloxacin-loaded solid lipid nanoparticles(CIP-SLNs) and to determine its antibacterial activity. Methods Ciprofloxacin-loaded nanoparticles were prepared by emulsification and low temperature solidification methods, with cholesterol as lipid and Tween-80 as surfactant. The particle size, Zeta potential, drug loading, encapsulation efficiency, dispersion and sustained release in vitro of CIP-SLNs were investigated with transmission electron microscopy and ultraviolet-visible spectroscopy, respectively. The minimum inhibitory concentration of drugs against E.coil were determined by double dilution method. Results Transmission electron microscopy showed that CIP-SLNs was spherical with a diameter of 40-70nm; Zeta potential was (21.8±1.3)mV; encapsulation efficiency was 77.54%; the high drug loading efficiency was 31.10%; the characteristic absorption peak of ciprofloxacin at 280nm was observed by ultraviolet-visible spectroscopy in nanoparticles; the sustained release in vitro for 72h was 78.6%. The minimum inhibitory concentration of CIP-SLNs against E.coil was 0.8μg/mL and that of ciprofloxacin was 1.6μg/mL. Conclusion Ciprofloxacin-loaded solid lipid nanoparticles have been successfully prepared by emulsification and low temperature solidification method, which enhances the antibacterial effect of ciprofloxacin. |
| Key words: ciprofloxacin solid lipid nanoparticles characterization minimum inhibitory concentration |
