| 引用本文： |
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张瑞琴,林思思,陈春球,等.JNK信号通路在小鼠术后肠麻痹发病机制中的作用[J].同济大学学报（医学版）,2019,40(1):34-38. [点击复制]
- ZHANG Rui-qin,LIN Si-si,CHEN Chun-qiu,et al.Roles of JNK signaling pathway in murine postoperative ileus[J].同济大学学报（医学版）,2019,40(1):34-38. [点击复制]
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| 摘要: |
| 目的 探讨JNK信号通路在小鼠术后肠麻痹（POI）发病机制中的作用。方法 将野生型C57/BL6小鼠（WT）及同品系的JNK-/-小鼠均随机分成假手术组（Sham组，n=6）和肠麻痹组（POI组，n=6）。采用经典小肠操作方法诱导POI模型，术后24h给小鼠碳末灌胃，20min后麻醉小鼠，开腹取小肠评估肠动力，取回肠评估组织学改变，检测髓过氧化物酶（MPO）、IL-1β、IL-6水平及Claudin-2蛋白表达。结果 与Sham组小鼠相比，无论WT或JNK-/-小鼠其POI组的小肠排推率（分别为21%与33%）均明显降低（P=0.034及P=0.045，均P<0.05），小肠组织MPO活性水平（分别为0.608U/g与0.433U/g）明显升高（均P<0.05）；与WT小鼠POI组比较，JNK-/-小鼠POI组的小肠运动功能及其组织病理变化有所改善，炎症介质如MPO、IL-1β及IL-6水平均有明显降低（均P<0.05），小肠Claudin-2蛋白表达也降低（P<0.01）。结论 JNK基因敲除减轻小鼠肠道炎症反应、改善POI，表明JNK信号通路参与POI的发病过程。 |
| 关键词: 术后肠麻痹 小鼠 炎症介质 JNK信号通路 |
| DOI：10.16118/j.1008-0392.2019.01.007 |
| 投稿时间：2018-10-18 |
| 基金项目:国家自然科学基金(31571181)；上海市卫计委科研课题(201540382) |
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| Roles of JNK signaling pathway in murine postoperative ileus |
| ZHANG Rui-qin,LIN Si-si,CHEN Chun-qiu,LI Kun,LI Yong-yu |
| (Dept. of Pathophysiology-Institute of Digestive Disease, Medical College, Tongji University, Shanghai 200092, China; Dept. of Pathology, West Branch of The Second Hospital, Shanxi Medical University, Taiyuan 030053, Shanxi Province, China;Dept. of Surgery, Tenth People’s Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To explore the roles of JNK signaling pathway in the murine postoperative ileus(POI). Methods The wild-type (WT) C57/BL6 mice and JNK knockout(JNK-/-) mice of the same strain were randomly divided into sham operation group(Sham group, n=6) and POI group(n=6). The POI model was induced by the classical small bowel manipulation. Mice were treated with a charcoal marking mixture by oral gavage after 24h and then sacrificed with anesthesia 20 min later. The whole small intestines were harvested. The intestinal motility and the histological changes of the ileum were evaluated, the levels of myeloperoxidase(MPO), interleukin-1β(IL-1β) and IL-6 in ileum were measured, the expression of Claudin-2 protein was determined. Results Compared with the sham group, the gastrointestinal transit was significantly decreased(P<0.05), and the MPO activity level of the small intestine was significantly increased(P<0.05) both in the WT mice and JNK-/-mice POI groups. Compared with WT POI mice, the gastrointestinal transit and the intestinal pathological changes were improved, and the levels of MPO, IL-1β and IL-6, and the expression of Claudin-2 protein in the intestinal tissue were significantly decreased(P<0.05 or P<0.01) in JNK-/-POI mice. Conclusion The knockout of JNK gene alleviates intestinal inflammation in mice and improves POI, indicating that JNK signaling pathway may be involved in the pathogenesis of POI. |
| Key words: postoperative ileus mice inflammatory factors JNK signaling pathway |
