| 引用本文： |
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朱 勇,高 浩,贺其志,等.宫颈脱落细胞蜡块在宫颈病变初筛中的作用[J].同济大学学报（医学版）,2018,39(5):54-59. [点击复制]
- ZHU Yong,GAO Hao,HE Qin-zhi,et al.Application of cervical exfoliated cell wax block in preliminary screening of cervical lesions[J].同济大学学报（医学版）,2018,39(5):54-59. [点击复制]
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| 摘要: |
| 目的 探索价格低廉、制片简便且可长期保存的宫颈脱落细胞蜡块制片在宫颈病变初筛中的临床意义。方法 收集宫颈病理诊断与新柏氏液基细胞学技术（thinprep cytologic test, TCT）诊断相符的TCT剩余样本，分为未见上皮内病变或恶性细胞(negative for intraepithelial lesion or malignancy, NILM)、低级别鳞状上皮内病变(low-grade squamous intraepithelial lesion, LSIL)、高级别鳞状上皮内病变(high-grade squamous intraepithelial lesion, HSIL)及鳞状细胞癌(squamous cell carcinoma of cervix, SCC)各30例，将同一样本分别进行TCT制片及细胞蜡块制片，H-E染色法、免疫组织化学法（mmunohistochemistry, IHC）分别检测Ki-67、P16INK4的表达，分析细胞蜡块制片对比TCT制片在宫颈病变初筛中的临床意义。结果 宫颈脱落细胞蜡块制作的成功率约90.8%，H-E染色病理阅片满意度不差于TCT制片；IHC检测Ki-67、P16INK4的表达与TCT制片差异无统计学意义（P＞0.05）。结论 宫颈脱落细胞蜡块制片用于宫颈病变初筛的技术方法具有临床可行性，结合其优势可考虑替代TCT制片在临床推广。 |
| 关键词: 宫颈病变 细胞蜡块 新柏氏液基细胞学技术 筛查 |
| DOI：10.16118/j.1008-0392.2018.05.011 |
| 投稿时间：2018-01-17 |
| 基金项目:国家自然科学基金面上项目（81372305);上海市卫计委重点项目（201540386) |
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| Application of cervical exfoliated cell wax block in preliminary screening of cervical lesions |
| ZHU Yong,GAO Hao,HE Qin-zhi,MEILIGULI·Mo-hemaiti,WANG Kai,GUO Xiao-qing |
| (Shihezi University School of Medicine, Shihezi 832003, Xinjiang, Urgur Autonomous Region, China;Dept. of Obstetrics and Gynecology, First Maternity and Infant Hospital, Tongji University, Shanghai 200092, China;Dept. of Pathology, First Maternity and Infant Hospital, Tongji University, Shanghai 200092, China;Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University, Shanghai 200092,China) |
| Abstract: |
| Objective To assess the application of cervical exfoliated cell wax block in preliminary screening of cervical lesions. Methods One hundred and twenty residual samples of cervical exfoliated cells with the consistent diagnosis between pathology and thinprep cytology test(TCT) were collected. There were 30 samples of each of the following diagnostic categories: negative intraepithelial lesion or malignancy(NILM), low-grade squamous intraepithelial lesion(LSIL), high-grade squamous intraepithelial lesion(HSIL) and squamous cell carcinoma of cervix(SCC). Bai-fluid-base TCT and cell wax block were prepared in the same sample, then hematoxylin-eosin(HE) staining and immunohistochemistry(IHC) were performed to detect the expression of Ki-67 and P16INK4. The application of cervical exfoliated cell wax block in screening of cervical lesions was evaluated by comparing with TCT result. Results The production rate of cervical exfoliated cell wax block was 90.8%, and the satisfaction degree of cervical exfoliated cell wax block was not worse than TCT. IHC showed that there was no significant difference in expression of Ki-67 and P16INK4 between TCT and cellular wax block(P>0.05). Conclusion The technique of cell wax block is clinically feasible, which may replace the TCT in preliminary screening cervical lesions in some extent. |
| Key words: cervical lesions cell wax block Bai-fluid-base TCT screening |
