| 引用本文： |
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刘宇,盛敏杰,李冰,等.MRL/lpr小鼠干眼模型的实验研究[J].同济大学学报（医学版）,2017,38(5):24-27. [点击复制]
- LIU Yu,SHENG Min-jie,LI Bing,et al.MRL/lpr mice as a dry eye mouse model[J].同济大学学报（医学版）,2017,38(5):24-27. [点击复制]
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| 摘要: |
| 目的 探讨患有自身免疫疾病的MRL/lpr小鼠作为临床干眼研究动物模型的科学性及实用性。方法8周龄MRL/lpr及BLAB/C雌性小鼠各15只，分为实验组、对照组。分别于8、12、16、20周对各组小鼠行酚红棉线实验检测泪液分泌情况；取各组小鼠泪腺组织行H-E染色分析其淋巴细胞浸润情况；Western印迹法检测泪腺炎症因子TNF-α和IL-1β的蛋白表达情况。结果实验组小鼠于16～20周时泪液分泌量显著少于对照组（P＜0.05），淋巴细胞浸润情况明显高于对照组（P＜0.05）,泪腺炎症因子TNF-α和IL-1β表达明显高于对照组（P＜0.05）。结论MRL/lpr小鼠是一种稳定、简单实用的干眼研究动物模型，可用于研究干眼炎症的发病机制。 |
| 关键词: MRL/lpr小鼠 干眼 动物模型 炎症因子 泪腺 |
| DOI：10.16118/j.1008-0392.2017.05.005 |
| 投稿时间：2017-02-28 |
| 基金项目:国家自然科学基金青年项目（81400373）；同济大学附属杨浦医院课题（Se1201521） |
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| MRL/lpr mice as a dry eye mouse model |
| LIU Yu,SHENG Min-jie,LI Bing,CHEN Li,LU Jia-jun,CHEN Yi-hui |
| (Dept. of Ophthalmology， Yangpu Hospital， Tongji University， Shanghai 200090, China) |
| Abstract: |
| Objective To examine whether the MRL/lpr mice with autoimmune lacrimal gland disease can be used as a dry eye mouse model. MethodsThirty 8-week-old female MRL/lpr mice (n=15) and BLAB/C mice (n=15) were used as experimental group and the control group, respectively. The animals were observed at 8, 12, 16 and 20 weeks. Cotton thread test was performed and the tear volume was measured. H-E staining was performed for lacrimal gland tissues and the infiltration of lymphocytes was analyzed. The expression of TNF-α and IL-1β in lacrimal gland was detected by Western blot. ResultsTear production was significantly decreased in the experimental group than that in the control group (P<0.05), especially at 16 and 20 weeks. HE staining showed more infiltrating lymphocytes in the experimental group in comparison with the control group. According to Western blot, the expression of inflammatory factors TNF-α and IL-1β in lacrimal gland was increased in experimental group（P＜0.05）. With the progression of the disease in MRL/lpr mice，the infiltrating lymphocytes and the TNF-α and IL-1β levels increased gradually（P＜0.05）. ConclusionMRL/lpr mice present the similar manifestations of human dry eye and the physiological and pathological changes in lacrimal gland, indicating that MRL/lpr mice can be used as a stable and feasible dry eye model. |
| Key words: MRL/lpr dry eye animal model inflammation cytokine lacrimal gland |
