| 引用本文： |
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袁华英,余韦臻,李宁丽,等.PM2.5对人皮肤成纤维细胞分泌IL-6、TGF-β1的影响[J].同济大学学报（医学版）,2017,38(3):35-39. [点击复制]
- YUAN Hua-ying,YU Wei-zhen,LI Ning-li,et al.Effect of PM2.5 on secretion of IL- 6 and TGF-β1 by human skin fibroblasts[J].同济大学学报（医学版）,2017,38(3):35-39. [点击复制]
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| 摘要: |
| 目的 探讨大气颗粒物PM2.5对人皮肤成纤维细胞(human skin fibroblast, HSF)分泌IL-6和TGF-β1的影响及胶原多肽(collagen polypeptide, CP)的干预作用。方法 将HSF分为PM2.5组和CP干预组。PM2.5组用0、25、50、100、200μg/ml的PM2.5与HSF培养48h；CP干预组用100μg/ml PM2.5和0、15、30、60μg/ml CP与HSF共培养48h。采用ELISA方法测定细胞上清液中IL-6、TGF-β1含量。结果 随着PM2.5浓度升高(0、25、50、100、200μg/ml),PM2.5组细胞上清液中的IL-6含量也逐渐升高,TGF-β1含量则逐渐下降,两者都存在一定的剂量-效应关系,各炎性因子浓度差异有统计学意义(P<0.05)；在PM2.5浓度为100μg/ml时,随着CP浓度升高(0、15、30、60μg/ml),PM2.5+CP组IL-6浓度逐渐下降,TGF-β1含量逐渐增加,两者都存在一定的剂量-效应关系,各炎性因子浓度差异有统计学意义(P<0.05)。结论 PM2.5能促进皮肤炎症,可能与上调IL-6、下调TGF-β1有关；而CP能通过抑制上述作用,缓解PM2.5对皮肤致炎作用。 |
| 关键词: PM2.5 人皮肤成纤维细胞 胶原多肽 白介素-6 转化生长因子β1 |
| DOI：10.16118/j.1008-0392.2017.03.007 |
| 投稿时间：2017-01-03 |
| 基金项目:上海市自然科学基金(162R1419200) |
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| Effect of PM2.5 on secretion of IL- 6 and TGF-β1 by human skin fibroblasts |
| YUAN Hua-ying,YU Wei-zhen,LI Ning-li,XU Hui |
| (Dept.of Dermatology, Shanghai Jiaotong University School of Medicine, Shanghai Ninth People's Hospital, Shanghai 200011, China;Shanghai Institute of Immunology, Shanghai 200025, China) |
| Abstract: |
| Objective To investigate the effect of PM2.5 on secretion of interleukin 6 (IL-6) and transforming growth factor β1 (TGF-β1) by human skin fibroblasts (HSF), and the protective effect of collagen polypeptide (CP) from insults of PM2.5. Methods Human skin fibroblasts were co-cultured with PM2.5 0,5, 50,0, 200μg/ml (PM2.5 groups) or PM2.5100μg/ml plus 0,5, 30,0μg/ml CP (PM2.5+CP groups) for 48h. The secretion of IL-6 and TGF-β1 were measured by ELISA method. Results With increasing concentration of PM2.5, the secretion of IL-6 increased and the secretion of TGF-β1 decreased gradually in a dose-effect manner (P<0.05). Compared to PM2.5 group, with increasing concentration of CP the secretion of IL-6 decreased and TGF-β1 increased gradually in PM2.5+CP groups in a dose-effect manner (P<0.05). Conclusion PM2.5 can induce skin inflammation, which is associated with up-regulation of IL-6 and down-regulation of TGF-β1, and CP can inhibit the effect of PM2.5 and alleviate the skin inflammation. |
| Key words: PM2.5 human skin fibroblast collagen polypeptide interleukin 6 transforming growth factor-β1 |
