GAO Yuan,LYU Yi-feng,MA Da-xi,et al.Impact of Roux-en-Y gastric bypass surgery on fatty liver in rats with high-fat diet-induced obesity and its mechanism[J].同济大学学报（医学版）,2017,38(3):25-29. [点击复制]
Impact of Roux-en-Y gastric bypass surgery on fatty liver in rats with high-fat diet-induced obesity and its mechanism
GAO Yuan,LYU Yi-feng,MA Da-xi,LI Zhi-hong
(Dept.of General Surgery, Tongji Hospital, Tongji University, Shanghai 200065, China;Dept.of General Surgery, Zhoupu Hospital, Pudong New Area, Shanghai 201318, China)
Abstract:
Objective To investigate the impact of Roux-en-Y gastric bypass surgery(RYGB) on fatty liver in rats with high-fat diet-induced obesity and its mechanism. Methods Thirty Wistar rats were randomly divided into three groups with 10 in each group. Rats in ND group were fed with normal diet, rats in HFD and HFD +RYGB groups were fed with high-fat diet; after 16 weeks, rats underwent sham operation in ND group and HFD group, and RYGB in HFD plus RYGB group. All rats were sacrificed after 4 weeks of operation. Total body weight, liver weight and visceral fat were measured. Serum triglyceride (TG), total cholesterol (TC), free fatty acid (FFA) concentrations were measured by ELISA. Hepatic tissues were harvested, pathological changes were observed by H-E and oil red O staining. The expressions of carbohydrate response element binding protein (ChREBP), sterol regulatory element-binding transcription factor 1c (SREBP-1c) and acetyl-CoA carboxylase (ACC) mRNA were detected by Real-time PCR. Results Total body weight, liver weight and visceral fat in HFD group were higher than those in ND group(P＜0.05); compared with HFD group, those indicators were decreased in HFD plus RYGB group(P＜0.05). Serum TG, TC, FFA concentrations in HFD group were higher than those in ND group(P＜0.05); compared with HFD group, those indicators were decreased in HFD plus RYGB group(P＜0.05). TG, TC concentrations in liver in HFD group were higher than those in ND group(P＜0.05); compared with HFD group, those indicators were decreased in HFD plus RYGB group(P＜0.05). H-E and oil red O staining showed that hepatic cell lipid, lipid vacuoles decreased in HFD plus RYGB group. Compared with HFD group, the expressions of ChREBP and SREBP-1c mRNA decreased in HFD plus RYGB group after 4 weeks of operation(P＜0.05). Conclusion RYGB can reduce visceral fat and blood lipid, alleviate the deposition of TG and TC in the liver, improve the fatty infiltration and vacuolar degeneration in the liver, down-regulate the expressions of ChREBP and SREBP-1c mRNA in rats with high-fat diet-induced obesity.
