| 引用本文： |
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王凯玲,费广茹,任涛.CpG-ODN对肺癌A549细胞化疗的协同作用[J].同济大学学报（医学版）,2017,38(3):14-18, 24. [点击复制]
- WANG Kai-ling,FEI Guang-ru,REN Tao.CpG-ODN enhances sensitivity to chemotherapy in lung cancer cell line A549[J].同济大学学报（医学版）,2017,38(3):14-18, 24. [点击复制]
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| 摘要: |
| 目的 研究CpG寡聚脱氧核苷酸(CpG-ODN)对肺癌A549细胞化疗的影响,并探讨其可能的作用机制。方法 将肺癌A549细胞分为空白对照组、DDP组、CpG-ODN组、DDP+CpG-ODN组、CpG-ODN+DDP组。采用CCK-8比色法检测细胞的增殖情况,流式细胞仪检测细胞凋亡情况,RT-PCR检测TLR9 mRNA的表达,Western印迹法检测TLR9信号通路中MyD88、AP-1的表达。结果 TLR9激活CpG ODN后,能显著提高细胞的增殖能力(P<0.05)；CpG-ODN与CpG-ODN+DDP两组细胞生长无明显差异(P>0.05)；CpG-ODN+DDP组细胞凋亡率较DDP组低(P<0.05),DDP+CpG-ODN组细胞凋亡率较DDP组明显升高(P<0.05)；DDP+CpG-ODN组TLR9 mRNA较DDP组明显升高(P<0.05)；DDP+CpG组AP-1蛋白表达上调(P<0.05)。结论 CpG-ODN激活TLR9信号通路后,可协同DDP促进细胞凋亡,提高顺铂化疗的敏感性。 |
| 关键词: 非小细胞肺癌 CpG寡聚脱氧核苷酸 化学疗法 |
| DOI：10.16118/j.1008-0392.2017.03.003 |
| 投稿时间：2017-03-06 |
| 基金项目:国家自然科学基金(81372347)；上海市浦东新区卫生系统重点学科建设项目(PWZx2014-10) |
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| CpG-ODN enhances sensitivity to chemotherapy in lung cancer cell line A549 |
| WANG Kai-ling,FEI Guang-ru,REN Tao |
| (Dept.of Respiratory Medicine, East Hospital, Tongji University, Shanghai 200120, China;Dept.of Respiratory Medicine, Anhui Provincial Hospital, Hefei 230001, Anhui Province, China) |
| Abstract: |
| Objective To investigate the effect of TLR9 activator CpG-ODN on chemosensitivity of lung cancer A549 cells. Methods Cultured lung cancer A549 cells were divided into control group, DDP group, CpG-ODN group, DDP+CpG-ODN group, CpG-ODN+DDP group. Cell proliferation was analyzed with CCK-8 method; cell apoptosis was examined with flow cytometry; the mRNA expression of TLR9 was detected with RT-PCR; and the protein expression of MyD88 and AP-1 was detected with Western blotting. Results The cell proliferation was improved in CpG-ODN groups(P<0.05); there was no significant difference between CpG-ODN and CpG-ODN+DDP groups(P>0.05). The apoptosis rate of CpG-ODN+DDP was lower than that of DDP(P<0.05), and the apoptotic rate of DDP+CpG-ODN was significantly higher than that of DDP(P<0.05). The expression of TLR9 mRNA in DDP+CpG-ODN was higher than that in DDP group(P<0.05). The expression of AP-1 in DDP+CpG group was up-regulated(P<0.05). Conclusion The data indicate that the CpG-ODN can increase cell apoptosis and enhance the sensitivity of lung cancer cells to cisplatin chemotherapy. |
| Key words: non-small cell lung cancer CpG-ODN chemotherapy |
