| 引用本文： |
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孙雅萍,吕立夏,徐国彤.应激伴侣分子敲除对MPTP诱导的帕金森模型小鼠的影响[J].同济大学学报（医学版）,2017,38(1):1-6. [点击复制]
- SUN Ya-ping,LYU Li-xia,XU Guo-tong.Effects of stress and charpone gene knock-out on neurological fuction in mice with mptp-induced Parkinson's disease[J].同济大学学报（医学版）,2017,38(1):1-6. [点击复制]
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| 摘要: |
| 目的研究应激伴侣分子(stress and charpone, STCH)基因敲除对MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)模型小鼠的影响。方法 对STCH基因敲除小鼠注射MPTP建模,对建模1、2、3周后小鼠进行转棒、强迫游泳、旷场实验、水迷宫行为学实验,观察建模小鼠的行为学差异；观察建模3周后小鼠TNF-α、IL-1β、IL-10、NGF等炎症因子及多巴胺(dopmaine, DA)、5-羟色胺(5-serotonin, 5-HT)等神经递质质含量变化。结果建模1、2周后,小鼠在运动、协调能力方面没有明显差异,但在一定程度上表现出抑郁症状；建模3周后,小鼠出现明显运动障碍,但其学习记忆、协调能力方面没有明显差异；小鼠体内炎症因子表达、DA与5-HT的含量差异无统计学意义。结论 STCH基因敲除可引起早期MPTP模型小鼠的抑郁,对其运动、学习能力没有显著影响。 |
| 关键词: 应激伴侣分子 MPTP 行为学 神经递质 |
| DOI：10.16118/j.1008-0392.2017.01.001 |
| 投稿时间：2016-09-18 |
| 基金项目:上海市卫生和计划生育委员会科研项目(201440229) |
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| Effects of stress and charpone gene knock-out on neurological fuction in mice with mptp-induced Parkinson's disease |
| SUN Ya-ping,LYU Li-xia,XU Guo-tong |
| (Institute of Clinical Vision Science, Medical College, Tongji Unveristy, Shanghai 200092, China) |
| Abstract: |
| Objective To investigate the effects of stress and charpone (STCH) gene knock-out on neurological function in mice with MPTP-induced parkinson's disease (PD). Methods MPTP(30mg/ml for 5d) was injected intraperitoneally in STCH +/- mice to induce the experimental PD model. Rotarod test, forced swimming test, open field test, morries water maze behavioural test were conducted in experimental and control groups 1,2 and 3 week after MPTP injecteion. The animals were sacrificed and brain samples were collected 3 week after MPTP injection. The mRNA experssion levels of TNF-α, IL-1β, IL-10 and NGF were tested by RT-PCR; the neurotransmitters dopamine and 5-serotonin levels were detected by HPLC-MS. Results Motor and coordination abilites results did not display significant differences between the MPTP + STCH(+/-) and STCH (+/-) mice at 1 or 2 week after modeling. But depression appeared in MPTP + STCH(+/-) mice during these time points. MPTP + STCH(+/-) mice appeared obvious motor disablities 3 week after modeling, while there were no significant differences in learing and coordination ablities between two groups. There were no significant differences in the expression of inflammatory factors and the contents of dopamine and 5-HT in brain tissue between the MPTP + STCH(+/-) and STCH (+/-) mice. Conclusion STCH gene knock-out may cause depression at early stage of MPTP-induced PD model, but may not affact motor and learning abilities. |
| Key words: stress and charpone MPTP behaviour tests neurotransmitters |
