| 引用本文： |
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张冰雨,曾瑞翔,舒晓亮,等.糖化LDL对人脐静脉血管内皮细胞SOD活性及基因表达的影响[J].同济大学学报（医学版）,2016,37(6):29-34. [点击复制]
- ZHANG Bing-yu,ZENG Rui-xiang,SHU Xiao-liang,et al.Effect of glycated LDL on SOD activity and gene expression of human umbilical vein endothelial cells[J].同济大学学报（医学版）,2016,37(6):29-34. [点击复制]
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| 摘要: |
| 目的 观察糖化低密度脂蛋白(gly-LDL)对人血管内皮细胞生长及抗氧化相关因子的影响,探讨糖尿病患者血管内皮损伤与gly-LDL的相关性及可能机制。方法 用完全培养基及不同浓度(G1组 0.06mg/L, G2组0.12mg/L, G3组0.24mg/L)的gly-LDL处理人脐静脉血管内皮细胞(HUVECs)24h、48h及72h,设对照组(Ctr),取细胞培养上清液检测SOD和MDA水平,同时用细胞划痕实验观察gly-LDL对内皮细胞迁移功能的影响,用实时半定量PCR(realtime quantitative PCR,RT-qPCR)方法测定Mn-SOD和eNOS的mRNA的表达情况。结果 与对照组相比不同浓度的gly-LDL均可抑制血管内皮细胞的迁移功能。较低或较高浓度的gly-LDL作用于人脐静脉内皮细胞时,随时间的增加SOD的活性及MDA的含量是升高的；中间浓度的gly-LDL作用于人脐静脉内皮细胞时,随时间的增加SOD活性是逐渐下降的,MDA含量是先增高后降低的。各组Mn-SOD的mRNA表达水平48h和72h均下调,eNOS的表达水平于24h和48h均下调,72h时上调。结论 Gly-LDL与糖尿病患者血管并发症可能存在一定的相关性,但不能肯定其是恶化因素之一。 |
| 关键词: 糖基化 低密度脂蛋白 人脐静脉血管内皮细胞 超氧化物歧化酶 丙二醛 |
| DOI：10.16118/j.1008-0392.2016.06.006 |
| 投稿时间：2016-06-28 |
| 基金项目:国家“863”高技术研究发展计划(2014AA022304) |
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| Effect of glycated LDL on SOD activity and gene expression of human umbilical vein endothelial cells |
| ZHANG Bing-yu,ZENG Rui-xiang,SHU Xiao-liang,LEI Tao |
| (Dept of Endocrine Tongji Hospital, Tongji University,Shanghai 200065, China;Dept.of Nutriology, Jinshan Hospital of Fudan University, Shanghai 201500, China;Dept.of Endocrinology, Putuo Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China) |
| Abstract: |
| Objective To investigate the effect of glycated low density lipoprotein(gly-LDL) on the growth and antioxidant related factors in endothelial cells, and investigate the correlation of gly-LDL with diabetes vascular endothelial injury and the possible mechanism. Methods Human umbilical vein endothelial cells(HUVECs) were treated with different concentrations(0.06mg/L, 0.12mg/L, 0.24mg/L) of gly-LDL for 24h, 48h and 72h. Superoxide dismutase(SOD) and malondialdehyde(MDA) levels in the cell culture supernatant were detected. The migrate function of endothelial cells was assessed by scratch test. Mn-SOD and eNOS mRNA expressions were determined by real-time quantitative PCR(RT-qPCR). Results Gly-LDL at different concentrations inhibited the migrate function of endothelial cells. When HUVCCs treated with 0.06mg/L and 0.24mg/L gly-LDL, the activity of SOD and MDA were increased with the time; while treated with 0.12mg/L gly-LDL, the SOD activity was decreases with the time and MDA was decrease after initial increase. Mn-SOD mRNA expression level were down-regulated after 48h and 72h in all gly-LDL treated groups. The expression of eNOS levels in 24h and 48h were down-regulated, but up-regulated in 72h. Conclusion Glycated LDL can inhibit growth of endothelial cells and change the expression of antioxidant related factors, which may be associated with the pathogenesis of vascular complications in diabetic patients. |
| Key words: glycosylation low density lipoprotein HUVECs superoxide dismutase malondialdehyde |
