LI Jun-wen,YANG Hong-yan,JIN Ling-jing,et al.Effect of aquaporin-4 on increased blood-brain barrier permeability during chronic hypoperfusion in rats[J].同济大学学报（医学版）,2016,37(5):14-19. [点击复制]
Objective To investigate the effect of the expression and configuration of aquaporin-4(AQP4) on permeability increasing of blood-brain barrier(BBB) during chronic hypoperfusion in rats. Methods Rat models of chronic hypoperfusion were established by bilateral common carotid artery occlusion(2-vessel occlusion, 2VO). BBB permeability was verified by evans blue(EB) content, the mRNA and protein expression of AQP4 was evaluated. OAPs was assessed by visualising the distribution and expression of AQP4 on astrocyte end-feet membranes. Results At early phase of postoperation, the permeability of BBB significantly increased compared with that in sham group(P<0.01),and the changing trends of mRNA and protein expression of AQP4 were consistent with the EB concentration. They were significantly increased to reach its maximal value compared with the sham rats and then gradually decreased at 1 week, 2 weeks and 1 month after 2VO, and returned to the control levels after 3 months. Double labelling with GFAP and AQP4 demonstrated that AQP4 was correctly anchored on astrocyte end-feet membranes, and was redistributed on membranes in the 3-day, 1-week and 2-week groups, and the GFAP and AQP4 were co-localized on astrocyte end-feet membranes after 3 months. Conclusion The permeability of BBB was increased in hypoperfusion rats, the expression and configuration change of AQP4 are closely related to the BBB permeability.
