LI Min,LIN Si-si,SHEN Li,et al.Effect of a novel cannabis preparation O-1602 on intestinal immunity and microflora in mice with acute pancreatitis[J].同济大学学报（医学版）,2016,37(5):1-5, 13. [点击复制]
Effect of a novel cannabis preparation O-1602 on intestinal immunity and microflora in mice with acute pancreatitis
LI Min,LIN Si-si,SHEN Li,ZHANG Rui-qin,LI Yong-yu
(Dept.of Pathophysiology, Institute of Digestive Disease, Medical College, Tongji University, Shanghai 200092, China;Dept.of Physiology, Zunyi Medical and Pharmaceutical College, Zunyi 563000, Guizhou Province, China;Dept.of Pathogen Biology, Medical College, Tongji University , Shanghai 200092, China)
Abstract:
Objective To investigate effect of a novel cannabis preparation O-1602 on the intestinal immunity and microflora in mice with acute pancreatitis(AP). Methods Twenty one adult C57/BL6 mice were randomly divided into three group: normal control group(control, n=7), AP group(n=7), and O-1602 treated group(AP+O-1602, n=7). AP was induced by intraperitoneal injection of caerulein(50μg /kg). Drug vehicle or O-1602(10mg/kg) was given intraperitoneal injection to AP group or AP+O-1602 group respectively, and equivalent volume of normal saline and drug vehicle was given to the control group. The mice were sacrificed at 3h after the last injection, and the blood, the whole small intestine and colon, the intestinal Peyer's patches were harvested. The microflora in the ileum and colon were examined； the T lymphocytes and its subgroups in the Peyer's patches were determined. The plasma levels of amylase, interleukin-10(IL-10) and monocyte chemotactic protein 1(MCP-1) were measured. Results Compared to the control group, mice in AP group presented with significantly increased intestinal microflora, plasma amylase activity and levels of IL-10 and MCP-1(P＜0.05 or P＜0.01). O-1602 attenuated these changes to some extent(P＜0.05). There was no significant difference in the numbers of Peyer's patches in the intestine among the three groups； but the percentage of CD3+ , CD4+ T lymphocyte decreased significantly in AP group and AP+O-1602 group(P＜0.01 and P＜0.05, respectively), and an indistinctively decreased percentage of CD4+/ CD8+ was observed. Conclusion O-1602 protects mice from cearulein-induced acute pancreatitis, which may be related to suppressing inflammatory response, promoting bacteria removal, and regulating T lymphocyte function in intestinal barrier.
