| 摘要: |
| 目的 评估线性探针技术快速检测耐多药结核分枝杆菌的效果。方法 选取317例结核分枝杆菌分离株分别进行MGIT960液体药物敏感性试验和线性探针快速检测,以液体法作为金标准,探讨线性探针检测技术的敏感性和特异性以及Kappa值。结果 317株结核分枝杆菌复合群样本分别进行MGIT960液体药物敏感性试验和线性探针快速检测,其中液体法利福平(RFP)耐药率为5.5%,异烟肼耐药率为11.5%,耐多药(MDR)患者检出率为4.7%；线性探针快速检测法利福平(RFP)耐药率为7.4%,异烟肼耐药率为11.7%,MDR检出率为5.7%。以液体法为金标准,线性探针检测技术检测利福平和异烟肼的敏感性和特异性分别为94.3％、97.7％和86.3％、98.0％。结论 线性探针检测技术是一个敏感、特异和快速的诊断MDR的有效方法。 |
| 关键词: 线性探针 结核分枝杆菌 耐多药 快速诊断 |
| DOI：10.16118/j.1008-0392.2015.06.022 |
| 投稿时间：2015-02-27 |
| 基金项目: |
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| Effect evaluation of Mycobacterium tuberculosis using linear probe technique |
| GAO Rong-liang,CHEN Jin |
| (Dept. of Clinical Laboratory, Shanghai Pulmonary Hospital, Shanghai Tongji University, Shanghai 200433, China) |
| Abstract: |
| Objective To evaluate the application of linear probe technology for rapid detection of multidrug-resistant Mycobacterium tuberculosis(MDR-TB) isolates. Methods Three hundred seve-nteen Mycobacterium tuberculosis isolates were tested for drug susceptibility by using MGIT 960 liquid method and linear probes technique. With liquid method as gold standard, the sensitivity, specificity and the Kappa value of linear probing method for detection of MDR-TB was evaluated. Result Both MGIT 960 drug susceptibility tests and linear probe detection were performed for a total of 317 Mycobacterium tuberculosis isolates. The MGIT 960 tests showed that the resistant rate to Rifampicin was 5.5%, Isoniazid was 11.5% and the rate of MDR-TB was 4.7%; while linear probe detection showed the resistance rate to Rifampicin was 7.4%, to Isoniazid was 11.7% and the rate of MDR-TB was 5.7%. With MGIT 960 method as golden standard, the sensitivities of linear probe method for detection of resistance to Rifampicin and Isoniazid were 94.3%, 97.7% and the specifici-ties were 86.3% and 98%, respectively. Conclusion Linear probe technique is a rapid and effective method for detection of MDR-TB. |
| Key words: linear probe technology Mycobacterium tuberculosis multidrug-resistant rapid diagnosis |
