| 引用本文： |
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邱裕友,唐翠松,胡剑,等.催产素干预兔骨质疏松模型骨微结构变化规律的实验研究[J].同济大学学报（医学版）,2015,36(5):23-28. [点击复制]
- QIU Yu-you,TANG Cui-song,HU Jian,et al.Effect of oxytocin on dynamic changes of bone mass in rabbit osteoporosis model[J].同济大学学报（医学版）,2015,36(5):23-28. [点击复制]
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| 摘要: |
| 目的 采用显微CT(Micro-CT)评估催产素(oxytocin, OT)干预骨质疏松(osteoporosis, OP)后的骨质及骨微结构时序变化规律。 方法 60只20周龄新西兰雌性大白兔随机分为对照组、OP模型组和OT干预组,每组20只。对照组施行假手术,OP组行双侧卵巢切除术(ovariectomy, OVX)。OT组在兔OVX术后第2周起,每天皮下注射催产素持续8周或至实验时间点为止,对照组和OP组则皮下注射生理盐水。三组分别在术后第0、4、8、10、12周处死,获取兔左后股骨上段行Micro-CT扫描,计测骨组织矿物质密度(tissue mineral density, TMD)和主要骨微结构参数,三组间结果进行比较、统计学分析。结果 OP组TMD、骨体积分数(bone volume fraction, BV/TV)、骨小梁数目(trabecular number, Tb.N)、骨小梁厚度(trabecular thickness, Tb.Th)从第8周开始较对照组明显降低(P＜0.05),骨结构模型指数(structure model index, SMI)、骨小梁间隙(trabecular space, Tb.Sp)则较对照组明显升高(P＜0.05)。OT组TMD直到第12周较对照组明显降低(P＜0.05),而BV/TV、Tb.N从第8周开始较OP组明显增加(P＜0.05),Tb.Th从第10周开始较OP组增加(P＜0.05),SMI、Tb.Sp从第8周开始较OP组明显降低(P＜0.05)。结论 OP模型兔早期活体OT干预可有效减缓骨质衰败程度,Micro-CT能动态观察其病理生理变化过程,OT可能是一种有效防治OP的药物。 |
| 关键词: 骨质疏松 显微CT 骨组织矿物质密度 催产素 |
| DOI：10.16118/j.1008-0392.2015.05.005 |
| 投稿时间：2015-04-21 |
| 基金项目:国家自然科学基金(81071134,81371517) |
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| Effect of oxytocin on dynamic changes of bone mass in rabbit osteoporosis model |
| QIU Yu-you,TANG Cui-song,HU Jian,WU Xing,HUA Ting,TANG Guang-yu |
| (Dept. of Radiology, Tenth People's Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To investigate the effect of oxytocin(OT) on dynamic changes of bone mass and bone microstructure in a rabbit osteoporosis(OP) model. Methods Sixty 20-week-old female New Zealand rabbits were randomly assigned into OT, OP and sham groups with 20 animals in each group. Rabbits in OT and OP groups were subjected to bilateral ovariectomy(OVX), and animals in sham group received sham operation. The rats were injected with vehicle daily for two weeks after operation. Rabbits in OT group were subcutaneously injected with OT 1mg/(kg·d) from wk 2 after OVX for 8 wks or till sacrifice; and rabbits in OP and sham groups received subcutaneous injection of normal saline. The left proximal femurs of the rabbits were examined with micro-CT at wks 0,4, 8,0, and 12 after operation. Differences in micro-CT structural parameters among the groups were analyzed using two-way ANOVA and Bonferroni post-hoc test. ResultsThe tissue mineral density(TMD), Tb.N, and Tb.Th values in OP group decreased significantly, while SMI, Tb.Sp values increased significantly from wk 8 compared with those in control group. The TMD value of OT group did not decreased significantly until wk 12, while BV/TV, Tb.N values increased from wk 8 compared with those of OP group. The SMI and Tb.Sp values decreased significantly from wk 8 compared with that of OP group. Conclusion Early intervention of oxytocin can attenuate bone degeneration in rabbits with osteoporosis. Micro-CT can dynamically assess the pathological changes of the bone degeneration of osteoporosis. |
| Key words: osteoporosis micro-CT tissue mineral density oxytocin |
